CMV enhancer-promoter is preferentially active in exocrine cells in vivo

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Abstract

The CMV enhancer-promoter sequence is often used as a transcriptional regulatory element in vector systems. We have used this control element to drive expression of GFP in a lentivirus vector transgene in pigs and chickens. Promoted as a 'universal' enhancer/promoter element capable of transcriptional activity in a number of cells in vitro, CMV-GFP transgene expression in vivo is preferentially observed in exocrine cells. This expression profile validates the use of this transcriptional control sequence to target expression to exocrine cells in gene transfer strategies.
Original languageEnglish
Pages (from-to)309-14
Number of pages6
JournalTransgenic Research
Volume18
Issue number2
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Animals
  • Animals, Genetically Modified
  • Chickens
  • Cytomegalovirus
  • Enhancer Elements, Genetic
  • Gene Transfer Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Humans
  • Lentivirus
  • NF-kappa B
  • Promoter Regions, Genetic
  • Swine
  • Transcription, Genetic
  • Transgenes

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