Abstract / Description of output
The CMV enhancer-promoter sequence is often used as a transcriptional regulatory element in vector systems. We have used this control element to drive expression of GFP in a lentivirus vector transgene in pigs and chickens. Promoted as a 'universal' enhancer/promoter element capable of transcriptional activity in a number of cells in vitro, CMV-GFP transgene expression in vivo is preferentially observed in exocrine cells. This expression profile validates the use of this transcriptional control sequence to target expression to exocrine cells in gene transfer strategies.
Original language | English |
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Pages (from-to) | 309-14 |
Number of pages | 6 |
Journal | Transgenic Research |
Volume | 18 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2009 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Animals, Genetically Modified
- Chickens
- Cytomegalovirus
- Enhancer Elements, Genetic
- Gene Transfer Techniques
- Genetic Vectors
- Green Fluorescent Proteins
- Humans
- Lentivirus
- NF-kappa B
- Promoter Regions, Genetic
- Swine
- Transcription, Genetic
- Transgenes