Co-operativity between modules within a C3b-binding site of complement receptor type 1

MD Kirkitadze, DTF Dryden, SM Kelly, NC Price, X Wang, M Krych, JP Atkinson, PN Barlow

Research output: Contribution to journalArticlepeer-review


Complement receptor type 1 (CR1) has 30 modules in its extracellular portion. An understanding of structure-function relationships,Within CR1 is being assembled gradually from studies of overlapping protein fragments. A CRI fragment corresponding to modules 16 and 17 was expressed recombinantly as a non-glycosylated protein and its stability and unfolding characteristics studied using biophysical techniques. The results were compared with data collected previously on a CR1 fragment encompassing modules 15, 16 and 17 which together constitute a C3b-binding site (Kirkitadze, M.D., Krych, M., Uhrin, D., Dryden, D.T.F., Smith, B.O., Wang, X., Hauhart, R., Atkinson, J.P. and Barlow, P.N. (1999) Biochemistry 38, 7019-7031). Modules within CR1 were found to cooperate during unfolding. The folding, stability and flexibility of this protein is therefore likely to be a complex function, and not just the sum, of contributions from individual modules. (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalFEBS Letters
Issue number1
Publication statusPublished - 1 Oct 1999


Dive into the research topics of 'Co-operativity between modules within a C3b-binding site of complement receptor type 1'. Together they form a unique fingerprint.

Cite this