Codon Usage and Splicing Jointly Influence mRNA Localization

Christine Mordstein, Rosina Savisaar, Robert S. Young, Jeanne Bazile, Lana Talmane, Juliet Luft, Michael Liss, Martin S. Taylor, Laurence D. Hurst, Grzegorz Kudla

Research output: Contribution to journalArticlepeer-review

Abstract

In the human genome, most genes undergo splicing, and patterns of codon usage are splicing dependent: guanine and cytosine (GC) content is the highest within single-exon genes and within first exons of multi-exon genes. However, the effects of codon usage on gene expression are typically characterized in unspliced model genes. Here, we measured the effects of splicing on expression in a panel of synonymous reporter genes that varied in nucleotide composition. We found that high GC content increased protein yield, mRNA yield, cytoplasmic mRNA localization, and translation of unspliced reporters. Splicing did not affect the expression of GC-rich variants. However, splicing promoted the expression of AT-rich variants by increasing their steady-state protein and mRNA levels, in part through promoting cytoplasmic localization of mRNA. We propose that splicing promotes the nuclear export of AU-rich mRNAs and that codon- and splicing-dependent effects on expression are under evolutionary pressure in the human genome.

Original languageEnglish
Pages (from-to)351-362.e8
Number of pages12
JournalCell Systems
Volume10
Issue number4
Early online date9 Apr 2020
DOIs
Publication statusPublished - 22 Apr 2020

Keywords

  • codon usage
  • synthetic biology
  • saturation mutagenesis
  • mRNA export
  • splicing
  • evolution

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