Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei

Appolinaire Djikeng*, Sylvine Raverdy, Jeremy Foster, Daniella Bartholomeu, Yinhua Zhang, Najib M. El-Sayed, Clotilde Carlow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Glycolysis and gluconeogenesis are, in part, driven by the interconversion of 3- and 2-phosphoglycerate (3-PG and 2-PG) which is performed by phosphoglycerate mutases (PGAMs) which can be cofactor dependant (dPGAM) or cofactor independent (iPGAM). The African trypanosome, Trypanosoma brucei, possesses the iPGAM form which is thought to play an important role in glycolysis. Here, we report on the use of RNA interference to down-regulate the T. brucei iPGAM in procyclic form T. brucei and evaluation of the resulting phenotype. We first demonstrated biochemically that depletion of the steady state levels of iPGM mRNA correlates with a marked reduction of enzyme activity. We further show that iPGAM is required for cell growth in procyclic T. brucei.

Original languageEnglish
Pages (from-to)887-892
Number of pages6
JournalParasitology Research
Volume100
Issue number4
DOIs
Publication statusPublished - Mar 2007

Keywords

  • RNA INTERFERENCE
  • BACILLUS-STEAROTHERMOPHILUS
  • GENOME
  • COMPARTMENTATION
  • GLYCOLYSIS
  • METABOLISM
  • EXPRESSION
  • GENERATION
  • MECHANISM
  • SEQUENCE

Cite this