Coincident sequence cloning

A. J. Brookes, D. J. Porteous

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a novel method, Coincident Sequence Cloning (CSC), which permits the selective recovery of common sequences shared between two complex and partially coincident DNA mixtures. We evaluate this method by integrating human DNA with DNA from a mouse-human somatic cell hybrid, and we recover exclusively human DNA products which are all represented in the hybrid genome. CSC strategies should be useful in addressing many highly complex problems in genome analysis.
Original languageEnglish
Pages (from-to)2609-2613
Number of pages5
JournalNucleic Acids Research
Volume19
Issue number10
Publication statusPublished - 1991

Keywords

  • Animals Base Sequence Blotting, Southern Cloning, Molecular/*methods DNA/isolation & purification Evaluation Studies as Topic Humans Hybrid Cells Mice Molecular Sequence Data Polymerase Chain Reaction

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