Projects per year
Abstract
The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we have used micro-contact printed arrays on glass slides presenting 190 combinations of 19 extracellular matrix molecules selected on the basis of their expression during embryonic development of the ventral midbrain. Using long-term neuroepithelial stem cells (Lt-NES), this approach identified a number of matricellular proteins that enhanced differentiation, with the combination of Sparc, Sparc-like (Sparc-l1) and Nell2 increasing the number of tyrosine hydroxylase+ neurons derived from Lt-NES cells and, critically for further translation, human pluripotent stem cells.
Original language | English |
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Journal | Frontiers in Cell and Developmental Biology |
Volume | 9 |
Early online date | 1 Dec 2021 |
DOIs | |
Publication status | E-pub ahead of print - 1 Dec 2021 |
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Dive into the research topics of 'Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells'. Together they form a unique fingerprint.Projects
- 2 Finished
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What are the mechanisms by which myelination in theCNS is established and refined?
1/03/15 → 30/11/20
Project: Research