Combined fluorescence lifetime and surface topographical imaging of biological tissue

Charlotte Hopkinson; Andrew Matheson; Neil Finlayson; Michael G. Tanner; Ahsan R Akram; Robert K. Henderson

doi:10.1364/BOE.504309

Combined fluorescence lifetime and surface topographical imaging of biological tissue

Charlotte Hopkinson, Andrew Matheson, Neil Finlayson, Michael G. Tanner, Ahsan R Akram, Robert K. Henderson

Research output: Contribution to journal › Article › peer-review

Abstract

In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface

Original language	English
Pages (from-to)	212-221
Number of pages	10
Journal	Biomedical Optics Express
Volume	15
Issue number	1
Early online date	14 Dec 2023
DOIs	https://doi.org/10.1364/BOE.504309
Publication status	E-pub ahead of print - 14 Dec 2023

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10.1364/BOE.504309Licence: Creative Commons: Attribution (CC-BY)

Combined fluorescence lifetime and surface topographical imaging of biological tissueAccepted author manuscript, 1.54 MB

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M. (Principal Investigator), Bradley, M. (Principal Investigator), Dhaliwal, K. (Co-investigator), Dhaliwal, K. (Co-investigator), Haslett, C. (Co-investigator), Henderson, R. (Co-investigator), Walsh, T. (Co-investigator) & Walsh, T. (Co-investigator)
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research

Combined fluorescence lifetime and surface topographical imaging of biological tissue
Matheson, A. (Creator), Finlayson, N. (Creator), Akram, A. (Creator), Henderson, R. (Creator), Tanner, M. G. (Creator) & Hopkinson, C. (Creator), Edinburgh DataShare, 10 Nov 2023
DOI: 10.7488/ds/7541
Dataset

Cite this

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title = "Combined fluorescence lifetime and surface topographical imaging of biological tissue",

abstract = "In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface",

author = "Charlotte Hopkinson and Andrew Matheson and Neil Finlayson and Tanner, {Michael G.} and Akram, {Ahsan R} and Henderson, {Robert K.}",

year = "2023",

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doi = "10.1364/BOE.504309",

language = "English",

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AU - Hopkinson, Charlotte

AU - Matheson, Andrew

AU - Finlayson, Neil

AU - Tanner, Michael G.

AU - Akram, Ahsan R

AU - Henderson, Robert K.

PY - 2023/12/14

Y1 - 2023/12/14

N2 - In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface

AB - In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface

U2 - 10.1364/BOE.504309

DO - 10.1364/BOE.504309

M3 - Article

SN - 2156-7085

VL - 15

SP - 212

EP - 221

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 1

ER -

Combined fluorescence lifetime and surface topographical imaging of biological tissue

Abstract

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Projects

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

Datasets

Combined fluorescence lifetime and surface topographical imaging of biological tissue

Cite this