Combined impedance spectroscopy and Fourier domain optical coherence tomography to monitor cells in three-dimensional structures

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To assess non-invasively and in real time the three-dimensional organization of cells within porous matrices by combining Fourier Domain Optical Coherence Tomography (FDOCT) and Impedance Spectroscopy (IS).

Materials and Methods: Broadband interferences resulting from the recombination of in-depth light scattering events within the sample and light from a reference arm are measured as a modulation of the spectrum generated by a superluminescent laser diode (lambda o = 930nm, FWHM 90nm). Fourier transform allows in-depth localization of the scatterers, and the 3D microstructure of the sample is reconstructed by raster scanning. Simultaneously impedance spectroscopy is performed with a dielectric probe connected to an impedance analyzer to gather additional cellular information, and synchronized with FDOCT measurements.

Results: A combined IS-FDOCT system allowing an axial resolution of 5 micrometer in tissues and impedance measurements over the range 20MHz-1GHz has been developed. Alginate matrices have been characterized in terms of microstructure and impedance. Matrices seeded with adipose-derived stem cells have been monitored without the use of labeling agent.

Conclusions: We have developed a multimodality system that will be instrumental to non-invasively monitor changes in total cell volume fraction and infer cell-specific dielectric properties in 3D structure. (Int J Artif Organs 2010; 33: 238-43)

Original languageEnglish
Pages (from-to)238-243
Number of pages6
JournalInternational journal of artificial organs
Volume33
Issue number4
Publication statusPublished - Apr 2010

Keywords

  • Optical coherence tomography
  • Impedance spectroscopy
  • Non-invasive monitoring
  • Tissue engineering
  • Three-dimensional matrices
  • PASSIVE ELECTRICAL-PROPERTIES
  • RADIO-FREQUENCY PERMITTIVITY
  • ENDED COAXIAL LINE
  • DIELECTRIC-SPECTROSCOPY
  • BIOLOGICAL TISSUES
  • DIELECTROPHORESIS
  • PROLIFERATION
  • BIOTECHNOLOGY
  • LYMPHOCYTES
  • ENRICHMENT

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