Objectives: To assess non-invasively and in real time the three-dimensional organization of cells within porous matrices by combining Fourier Domain Optical Coherence Tomography (FDOCT) and Impedance Spectroscopy (IS).
Materials and Methods: Broadband interferences resulting from the recombination of in-depth light scattering events within the sample and light from a reference arm are measured as a modulation of the spectrum generated by a superluminescent laser diode (lambda o = 930nm, FWHM 90nm). Fourier transform allows in-depth localization of the scatterers, and the 3D microstructure of the sample is reconstructed by raster scanning. Simultaneously impedance spectroscopy is performed with a dielectric probe connected to an impedance analyzer to gather additional cellular information, and synchronized with FDOCT measurements.
Results: A combined IS-FDOCT system allowing an axial resolution of 5 micrometer in tissues and impedance measurements over the range 20MHz-1GHz has been developed. Alginate matrices have been characterized in terms of microstructure and impedance. Matrices seeded with adipose-derived stem cells have been monitored without the use of labeling agent.
Conclusions: We have developed a multimodality system that will be instrumental to non-invasively monitor changes in total cell volume fraction and infer cell-specific dielectric properties in 3D structure. (Int J Artif Organs 2010; 33: 238-43)
|Number of pages||6|
|Journal||International journal of artificial organs|
|Publication status||Published - Apr 2010|
- Optical coherence tomography
- Impedance spectroscopy
- Non-invasive monitoring
- Tissue engineering
- Three-dimensional matrices
- PASSIVE ELECTRICAL-PROPERTIES
- RADIO-FREQUENCY PERMITTIVITY
- ENDED COAXIAL LINE
- BIOLOGICAL TISSUES