Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes

Pierre Rome, Hiroyuki Ohkura

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract / Description of output

Studies using Drosophila have played pivotal roles in advancing our understanding of molecular mechanisms of mitosis throughout the past decades, due to the short generation time and advanced genetic research of this organism. Drosophila is also an excellent model to study female meiosis in oocytes. Pathways such as the acentrosomal assembly of the meiotic spindle in oocytes are conserved from fly to humans. Collecting and manipulating large Drosophila oocytes for microscopy and biochemistry is both time- and cost-efficient, offering advantages over mouse or human oocytes. Therefore, Drosophila oocytes serve as an excellent platform for molecular studies of female meiosis using a combination of genetics, microscopy and biochemistry. Here we describe key methods to observe the formation of the meiotic spindle either in fixed or in live oocytes. Moreover, biochemical methods are described to identify protein-protein interactions in vivo.
Original languageEnglish
Title of host publicationMitosis and Meiosis Part B
EditorsHelder Maiato, Melina Schuh
Place of PublicationCambridge
Number of pages12
ISBN (Print)9780128141427
Publication statusPublished - 1 Jul 2018

Publication series

NameMethods in Cell Biology

Keywords / Materials (for Non-textual outputs)

  • Drosophila
  • oocyte
  • spindle
  • microtubule
  • immunostaining
  • RNAi
  • fluorescent in situ hybridization
  • transgenesis
  • live-imaging
  • protein-protein interaction
  • biochemistry
  • immunoprecipitation


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