@inbook{b5ce3c4e8e4549cc8230b4a1a88b0bfe,
title = "Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes",
abstract = "Studies using Drosophila have played pivotal roles in advancing our understanding of molecular mechanisms of mitosis throughout the past decades, due to the short generation time and advanced genetic research of this organism. Drosophila is also an excellent model to study female meiosis in oocytes. Pathways such as the acentrosomal assembly of the meiotic spindle in oocytes are conserved from fly to humans. Collecting and manipulating large Drosophila oocytes for microscopy and biochemistry is both time- and cost-efficient, offering advantages over mouse or human oocytes. Therefore, Drosophila oocytes serve as an excellent platform for molecular studies of female meiosis using a combination of genetics, microscopy and biochemistry. Here we describe key methods to observe the formation of the meiotic spindle either in fixed or in live oocytes. Moreover, biochemical methods are described to identify protein-protein interactions in vivo.",
keywords = "Drosophila, oocyte, spindle, microtubule, immunostaining, RNAi, fluorescent in situ hybridization, transgenesis, live-imaging, protein-protein interaction, biochemistry, immunoprecipitation",
author = "Pierre Rome and Hiroyuki Ohkura",
note = "Chapter 12 - Permission obtained to upload the AAM to PURE and make it available Evidence attached 06/04/2018 EN - the Funcers Author Manuscript is available through PMC I have emailed them to confirm the licence 21/08/2018 EN",
year = "2018",
month = jul,
day = "1",
doi = "10.1016/bs.mcb.2018.03.026",
language = "English",
isbn = "9780128141427",
volume = "145",
series = "Methods in Cell Biology",
publisher = "Humana Press",
pages = "237--248",
editor = "Helder Maiato and Melina Schuh",
booktitle = "Mitosis and Meiosis Part B",
edition = "238454",
}