Comparative analysis of a conserved gene block from the genome of the members of the genus ictalurivirus

Andor Doszpoly, Mária Benko, Giuseppe Bovo, Scott E Lapatra, Balázs Harrach

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

OBJECTIVE: Partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (HVs). Acipenserid (Aci) HV-2, originating from the white sturgeon (Acipenser transmontanus), and ictalurid (Ic) HV-2, isolated from the black bullhead (Ameiurus melas), are recently approved species of the genus Ictalurivirus of the family Alloherpesviridae.

METHODS: An almost 8,000-base-pair fragment, spanning between the genes of the DNA polymerase and the ATPase subunit of the terminase, was sequenced from each virus.

RESULTS: The size, position and orientation of 2 partial and 3 full open reading frames, contained in the studied genome fragment, proved to be similar to their counterparts in IcHV-1, the type species of the genus Ictalurivirus. Thus, a well-conserved genus-specific gene block was identified. In the members of two other genera (Cyprinivirus and Batrachovirus) of the family Alloherpesviridae, no such gene block could be found; the location and orientation of the homologous genes showed significant divergence.

CONCLUSION: The results of phylogenetic calculations were in good agreement with the genome arrangements inasmuch as AciHV-2, IcHV-1 and -2 are monophyletic and separated from the lineages of the other two genera. The new sequence enabled the inclusion of a hitherto unassigned HV, that of the Australian pilchard, into a phylogenetic calculation.

Original languageEnglish
Pages (from-to)282-9
Number of pages8
JournalIntervirology
Volume54
Issue number5
DOIs
Publication statusPublished - 2011

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cluster Analysis
  • Conserved Sequence
  • DNA, Viral
  • DNA-Directed DNA Polymerase
  • Endodeoxyribonucleases
  • Fishes
  • Genes, Viral
  • Genome, Viral
  • Ictalurivirus
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Analysis, DNA
  • Sequence Homology
  • Viral Proteins

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