Abstract
OBJECTIVE: Partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (HVs). Acipenserid (Aci) HV-2, originating from the white sturgeon (Acipenser transmontanus), and ictalurid (Ic) HV-2, isolated from the black bullhead (Ameiurus melas), are recently approved species of the genus Ictalurivirus of the family Alloherpesviridae.
METHODS: An almost 8,000-base-pair fragment, spanning between the genes of the DNA polymerase and the ATPase subunit of the terminase, was sequenced from each virus.
RESULTS: The size, position and orientation of 2 partial and 3 full open reading frames, contained in the studied genome fragment, proved to be similar to their counterparts in IcHV-1, the type species of the genus Ictalurivirus. Thus, a well-conserved genus-specific gene block was identified. In the members of two other genera (Cyprinivirus and Batrachovirus) of the family Alloherpesviridae, no such gene block could be found; the location and orientation of the homologous genes showed significant divergence.
CONCLUSION: The results of phylogenetic calculations were in good agreement with the genome arrangements inasmuch as AciHV-2, IcHV-1 and -2 are monophyletic and separated from the lineages of the other two genera. The new sequence enabled the inclusion of a hitherto unassigned HV, that of the Australian pilchard, into a phylogenetic calculation.
Original language | English |
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Pages (from-to) | 282-9 |
Number of pages | 8 |
Journal | Intervirology |
Volume | 54 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2011 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cluster Analysis
- Conserved Sequence
- DNA, Viral
- DNA-Directed DNA Polymerase
- Endodeoxyribonucleases
- Fishes
- Genes, Viral
- Genome, Viral
- Ictalurivirus
- Molecular Sequence Data
- Phylogeny
- Sequence Analysis, DNA
- Sequence Homology
- Viral Proteins