Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics

Nicola Harrison; Richard J Harrison; Catherine A Kidner

doi:10.1371/journal.pone.0153248

Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics

Nicola Harrison, Richard J Harrison, Catherine A Kidner

Research output: Contribution to journal › Article › peer-review

Abstract

Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.

Original language	English
Article number	e0153248
Number of pages	17
Journal	PLoS ONE
Volume	11
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0153248
Publication status	Published - 8 Apr 2016

Keywords / Materials (for Non-textual outputs)

CHLOROPLAST GENOME
MAXIMUM-LIKELIHOOD
INVERTED REPEAT
ASIAN BEGONIA
SEQUENCE
DNA
EVOLUTION
GENUS
ORGANIZATION
RARE

Access to Document

10.1371/journal.pone.0153248

journal.pone.0153248Final published version, 4.24 MB

Cite this

@article{b6746b36f9aa47a098b18f677bd3124d,

title = "Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics",

abstract = "Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.",

keywords = "CHLOROPLAST GENOME, MAXIMUM-LIKELIHOOD, INVERTED REPEAT, ASIAN BEGONIA, SEQUENCE, DNA, EVOLUTION, GENUS, ORGANIZATION, RARE",

author = "Nicola Harrison and Harrison, \{Richard J\} and Kidner, \{Catherine A\}",

note = "BBSRC PhD student (Nikki) was funded by grant numbers BB/B511978/1 from 01/10/07 to 30/09/08 and from grant ref BB/D526210/1 from 01/10/08 to 31/08/11 (she went part-time, so the grant number changed).",

year = "2016",

month = apr,

day = "8",

doi = "10.1371/journal.pone.0153248",

language = "English",

volume = "11",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "4",

}

TY - JOUR

T1 - Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics

AU - Harrison, Nicola

AU - Harrison, Richard J

AU - Kidner, Catherine A

N1 - BBSRC PhD student (Nikki) was funded by grant numbers BB/B511978/1 from 01/10/07 to 30/09/08 and from grant ref BB/D526210/1 from 01/10/08 to 31/08/11 (she went part-time, so the grant number changed).

PY - 2016/4/8

Y1 - 2016/4/8

N2 - Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.

AB - Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.

KW - CHLOROPLAST GENOME

KW - MAXIMUM-LIKELIHOOD

KW - INVERTED REPEAT

KW - ASIAN BEGONIA

KW - SEQUENCE

KW - DNA

KW - EVOLUTION

KW - GENUS

KW - ORGANIZATION

KW - RARE

UR - https://www.scopus.com/pages/publications/84963747843

U2 - 10.1371/journal.pone.0153248

DO - 10.1371/journal.pone.0153248

M3 - Article

C2 - 27058864

SN - 1932-6203

VL - 11

JO - PLoS ONE

JF - PLoS ONE

IS - 4

M1 - e0153248

ER -

Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Data from: Comparative analysis of Begonia plastid genomes and their utility for species-level phylogenetics

Cite this

Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Datasets

Data from: Comparative analysis of Begonia plastid genomes and their utility for species-level phylogenetics

Cite this