Comparative analysis of c-kit gene expression and c-Kit immunoreactivity in horses with and without obstructive intestinal disease

C. Fintl, G.T. Pearson, I.G. Mayhew, C.S. Lowden, P.A. Hopwood, C.J. Palgrave, C.J. Proudman, G.B. Edwards, Sarah Taylor, N.P.H. Hudson

Research output: Contribution to journalArticlepeer-review

Abstract

Previous immunohistochemical studies targeting the receptor tyrosine kinase (c-Kit) have demonstrated an apparent reduction in the number of gastrointestinal pacemaker cells - the interstitial cells of Cajal (ICC) - in horses with intestinal motility disorders This study compared the level of transcription of the c-kit gene encoding this receptor in horses with and without such motility disorders Transcription levels of this gene were also compared to the density of ICC immunohistochemically positive for the c-Kit antigen Intestinal samples were collected from 18 horses with intestinal disease and from 15 control animals Following gene extraction and identification real time quantitative analysis of c kit and a control gene ACTB (beta-actin) was carried out on all samples and the density of the c-Kit-positive ICC compared There was a significant reduction in c-Kit immunoreactivity in the ICC of horses with large intestinal obstructive disorders relative to controls but no significant difference in the transcription of the c kit gene between normal and affected animals Further studies will be required to elucidate the mechanisms regulating c Kit expression and to assess the pathophysiological significance of these findings( C) 2009 Elsevier Ltd All rights reserved
Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalVeterinary Journal
Volume186
Issue number1
DOIs
Publication statusPublished - 2010

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