Comparative Analysis of Monocyte Subsets in the Pig

Lynsey Fairbairn, Ronan Kapetanovic, Dario Beraldi, David P Sester, Chris K Tuggle, Alan L Archibald, David A Hume

Research output: Contribution to journalArticlepeer-review

Abstract

Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14(hi)-CD163(low) and CD14(low)-CD163(hi), show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14(low)-CD163(high) cells are actually equivalent to intermediate human monocytes, and there is no CD14(-) CD16(+) "nonclassical" population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.
Original languageEnglish
Pages (from-to)6389-6396
JournalJournal of Immunology
Volume190
Issue number12
Early online date10 May 2013
DOIs
Publication statusPublished - Jun 2013

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