Projects per year
Abstract
Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14(hi)-CD163(low) and CD14(low)-CD163(hi), show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14(low)-CD163(high) cells are actually equivalent to intermediate human monocytes, and there is no CD14(-) CD16(+) "nonclassical" population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.
| Original language | English |
|---|---|
| Pages (from-to) | 6389-6396 |
| Journal | The Journal of Immunology |
| Volume | 190 |
| Issue number | 12 |
| Early online date | 10 May 2013 |
| DOIs | |
| Publication status | Published - Jun 2013 |
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Dive into the research topics of 'Comparative Analysis of Monocyte Subsets in the Pig'. Together they form a unique fingerprint.Projects
- 3 Finished
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Ark Genomics
Watson, M. (Principal Investigator), Archibald, A. (Co-investigator) & Burt, D. (Co-investigator)
1/04/12 → 31/03/17
Project: Research
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Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
1/04/12 → 31/03/17
Project: Research
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Transcriptional control of macrophage function in the pig and its relationship to infectious disease susceptibility
Hume, D. (Principal Investigator) & Archibald, A. (Co-investigator)
1/10/08 → 22/10/12
Project: Research