Projects per year
Abstract / Description of output
Cre/Lox technology is a powerful tool in the mouse genetics tool-box as it enables tissue-specific and inducible mutagenesis of specific gene loci. Correct interpretation of phenotypes depends upon knowledge of the Cre expression pattern in the chosen mouse driver line to ensure that appropriate cell types are targeted. For studies of the brain and neurological disease a pan-neuronal promoter that reliably drives efficient neuron-specific transgene expression would be valuable. Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of all neurons and weakly active in testes. In contrast the Snap25-IRES2-cre line expressed Cre in a high proportion of neurons (~85%) and was indetectable in all non-brain tissues that were analysed, including testes. Our findings suggest that for many purposes Snap25-IRES2-cre is superior to Syn1-cre as a potential pan-neuronal cre driver.
Original language | English |
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Number of pages | 14 |
Journal | Wellcome Open Research |
Volume | 7 |
Issue number | 185 |
DOIs | |
Publication status | Published - 8 Jul 2022 |
Keywords / Materials (for Non-textual outputs)
- cre driver mice
- cre-lox technology
- neurodevelopment
- synapsin1
- snap25
- MeCP2 reporter
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Dive into the research topics of 'Comparative analysis of potential broad-spectrum neuronal Cre drivers'. Together they form a unique fingerprint.Projects
- 3 Finished
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SynthSys-Mammalian: Edinburgh Mammalian Synthetic Biology Research Centre
14/11/14 → 31/03/22
Project: Research