Comparative analysis on the structural features of the 5' flanking region of kappa-casein genes from six different species

Akos Gerencsér, Endre Barta, Simon Boa, Petros Kastanis, Zsuzsanna Bösze, C Bruce A Whitelaw

Research output: Contribution to journalArticlepeer-review

Abstract

Kappa-casein plays an essential role in the formation, stabilisation and aggregation of milk micelles. Control of kappa-casein expression reflects this essential role, although an understanding of the mechanisms involved lags behind that of the other milk protein genes. We determined the 5'-flanking sequences for the murine, rabbit and human kappa-casein genes and compared them to the published ruminant sequences. The most conserved region was not the proximal promoter region but an approximately 400 bp long region centred 800 bp upstream of the TATA box. This region contained two highly conserved MGF/STAT5 sites with common spacing relative to each other. In this region, six conserved short stretches of similarity were also found which did not correspond to known transcription factor consensus sites. On the contrary to ruminant and human 5' regulatory sequences, the rabbit and murine 5'-flanking regions did not harbour any kind of repetitive elements. We generated a phylogenetic tree of the six species based on multiple alignment of the kappa-casein sequences. This study identified conserved candidate transcriptional regulatory elements within the kappa-casein gene promoter.
Original languageEnglish
Pages (from-to)117-28
Number of pages12
JournalGenetics Selection Evolution
Volume34
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • 5' Flanking Region
  • Animals
  • Binding Sites
  • Caseins
  • Cattle
  • Consensus Sequence
  • Conserved Sequence
  • Goats
  • Humans
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Promoter Regions, Genetic
  • Rabbits
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sheep
  • Transcription Factors

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