Comparison of real-time reverse transcriptase (RT)-PCR assays for detection of swine hepatitis E virus in fecal samples

Priscilla Freitas Gerber, Chao-Ting Xiao, Dianjun Cao, Xiang-Jin Meng, Tanja Opriessnig

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed for mHEV detection with great differences in sensitivity. The aim of this study was to compare the performance of two single-plex real-time reverse transcriptase (RT)-PCR assays for broad detection of all four mHEV genotypes (assays A and B) and two duplex real-time RT-PCR assays for detection and differentiation of mHEV genotypes 3 and 4 (assay C and D). RNAs extracted from 28 fecal samples from pigs experimentally inoculated with HEV genotype 3 and 186 fecal samples from commercial pigs with unknown HEV exposure were tested by all four assays. In experimental samples, HEV RNA was detected in 96.4% (assay A), 39.2% (assay B), 14.2% (assay C), and 0% (assay D) of the samples. In field samples with unknown HEV exposure, HEV RNA was detected in 67.2% (assay A), 36.4% (assay B), 1.1% (assay C), and 0.5% (assay D) of the samples. Assays showed an overall poor agreement (κ = 0.19 to 0.03) with differences in detection rates between assays (p
Original languageEnglish
Pages (from-to)1045-1051
JournalJournal of Clinical Microbiology
Volume52
Issue number4
Early online date15 Jan 2014
DOIs
Publication statusPublished - Apr 2014

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