Comparison of the activity of carp and rat β-actin gene regulatory sequences in tilapia and rainbow trout embryos

M. Samsul Alam, F. Louise Lavender, Arati Iyengar, M. Azizur Rahman, Hala H. Ayad, Richard Lathe, Steven D. Morley, Norman Maclean*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A comparative study on the level of expression of lacZ reporter constructs driven by equivalent carp and rat β-actin regulatory sequences was carried out in embryos of tilapia and rainbow trout. DNA was microinjected into fertilised tilapia and rainbow trout eggs and the embryos/fry were assayed at various developmental stages for β-galactosidase expression. We provide evidence to demonstrate that the carp β-actin promoter/lacZ reporter gene is expressed at higher levels than the equivalent rat β-actin construct in both species.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMolecular Reproduction and Development
Volume45
Issue number2
DOIs
Publication statusPublished - Oct 1996

Keywords / Materials (for Non-textual outputs)

  • Gene regulation
  • lacZ
  • Transgene expression
  • Transgenic fish
  • Transient expression

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