Abstract
A comparative study on the level of expression of lacZ reporter constructs driven by equivalent carp and rat β-actin regulatory sequences was carried out in embryos of tilapia and rainbow trout. DNA was microinjected into fertilised tilapia and rainbow trout eggs and the embryos/fry were assayed at various developmental stages for β-galactosidase expression. We provide evidence to demonstrate that the carp β-actin promoter/lacZ reporter gene is expressed at higher levels than the equivalent rat β-actin construct in both species.
Original language | English |
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Pages (from-to) | 117-122 |
Number of pages | 6 |
Journal | Molecular Reproduction and Development |
Volume | 45 |
Issue number | 2 |
DOIs | |
Publication status | Published - Oct 1996 |
Keywords / Materials (for Non-textual outputs)
- Gene regulation
- lacZ
- Transgene expression
- Transgenic fish
- Transient expression