Comparison of the activity of carp and rat beta-actin gene regulatory sequences in tilapia and rainbow trout embryos

M S Alam, F L Lavender, A Iyengar, M A Rahman, H H Ayad, R Lathe, Steven D Morley, N Maclean

Research output: Contribution to journalArticlepeer-review

Abstract

A comparative study on the level of expression of lacZ reporter constructs driven by equivalent carp and rat beta-actin regulatory sequences was carried out in embryos of tilapia and rainbow trout. DNA was microinjected into fertilised tilapia and rainbow trout eggs and the embryos/fry were assayed at various developmental stages for beta-galactosidase expression. We provide evidence to demonstrate that the carp beta-actin promoter/lacZ reporter gene is expressed at higher levels than the equivalent rat beta-actin construct in both species. (C) 1996 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMolecular Reproduction and Development
Volume45
Issue number2
Publication statusPublished - Oct 1996

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