Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction

Mohammed Sebaihia, Andrew Preston, Duncan J Maskell, Holly Kuzmiak, Terry D Connell, Natalie D King, Paul E Orndorff, David M Miyamoto, Nicholas R Thomson, David Harris, Arlette Goble, Angela Lord, Lee Murphy, Michael A Quail, Simon Rutter, Robert Squares, Steven Squares, John Woodward, Julian Parkhill, Louise M Temple

Research output: Contribution to journalArticlepeer-review

Abstract

Bordetella avium is a pathogen of poultry and is phylogenetically distinct from Bordetella bronchiseptica, Bordetella pertussis, and Bordetella parapertussis, which are other species in the Bordetella genus that infect mammals. In order to understand the evolutionary relatedness of Bordetella species and further the understanding of pathogenesis, we obtained the complete genome sequence of B. avium strain 197N, a pathogenic strain that has been extensively studied. With 3,732,255 base pairs of DNA and 3,417 predicted coding sequences, it has the smallest genome and gene complement of the sequenced bordetellae. In this study, the presence or absence of previously reported virulence factors from B. avium was confirmed, and the genetic bases for growth characteristics were elucidated. Over 1,100 genes present in B. avium but not in B. bronchiseptica were identified, and most were predicted to encode surface or secreted proteins that are likely to define an organism adapted to the avian rather than the mammalian respiratory tracts. These include genes coding for the synthesis of a polysaccharide capsule, hemagglutinins, a type I secretion system adjacent to two very large genes for secreted proteins, and unique genes for both lipopolysaccharide and fimbrial biogenesis. Three apparently complete prophages are also present. The BvgAS virulence regulatory system appears to have polymorphisms at a poly(C) tract that is involved in phase variation in other bordetellae. A number of putative iron-regulated outer membrane proteins were predicted from the sequence, and this regulation was confirmed experimentally for five of these.
Original languageEnglish
Pages (from-to)6002-15
Number of pages14
JournalJournal of Bacteriology
Volume188
Issue number16
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Animals
  • Bacterial Proteins
  • Base Sequence
  • Bordetella
  • Cell Surface Extensions
  • Chromosomes, Bacterial
  • Genetic Variation
  • Genome, Bacterial
  • Molecular Sequence Data
  • Poultry

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