Compendium of 4,941 rumen metagenome-assembled genomes for rumen microbiome biology and enzyme discovery

Robert Stewart; Marc D. Auffret; Amanda Warr; Alan Walker; Rainer Roehe; Michael Watson

doi:10.1038/s41587-019-0202-3

Compendium of 4,941 rumen metagenome-assembled genomes for rumen microbiome biology and enzyme discovery

Robert Stewart, Marc D. Auffret, Amanda Warr, Alan Walker, Rainer Roehe, Michael Watson

Research output: Contribution to journal › Article › peer-review

Abstract

Ruminants provide essential nutrition for billions of people worldwide. The rumen is a specialised stomach adapted to the breakdown of plant-derived complex polysaccharides, and collectively the rumen microbiota encode the thousands of enzymes responsible. Here we present a comprehensive analysis of over 6.5 terabytes of Illumina and Nanopore sequence data, including assembly of 4941 metagenome-assembled genomes, and several single-contig, whole-chromosome assemblies of novel rumen bacteria. We also present the largest dataset of predicted proteins from the rumen and provide rich annotation against public datasets. Together these data will form an essential part of future studies of rumen microbiome structure and function of the rumen microbiota

Original language	English
Pages (from-to)	953-961
Journal	Nature Biotechnology
Volume	37
Early online date	2 Aug 2019
DOIs	https://doi.org/10.1038/s41587-019-0202-3
Publication status	E-pub ahead of print - 2 Aug 2019

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s41587-019-0202-3Final published version, 4.06 MBLicence: Creative Commons: Attribution (CC-BY)

Understanding the functional and genomic architecture of the rumen microbiome affecting performance traits in bovines
Watson, M.
BBSRC
1/12/16 → 30/11/19
Project: Research

Mick Watson
- Global Academy of Agriculture and Food Systems
Person: Academic: Research Active

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AU - Stewart, Robert

AU - Auffret, Marc D.

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AU - Walker, Alan

AU - Roehe, Rainer

AU - Watson, Michael

PY - 2019/8/2

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AB - Ruminants provide essential nutrition for billions of people worldwide. The rumen is a specialised stomach adapted to the breakdown of plant-derived complex polysaccharides, and collectively the rumen microbiota encode the thousands of enzymes responsible. Here we present a comprehensive analysis of over 6.5 terabytes of Illumina and Nanopore sequence data, including assembly of 4941 metagenome-assembled genomes, and several single-contig, whole-chromosome assemblies of novel rumen bacteria. We also present the largest dataset of predicted proteins from the rumen and provide rich annotation against public datasets. Together these data will form an essential part of future studies of rumen microbiome structure and function of the rumen microbiota

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JO - Nature Biotechnology

JF - Nature Biotechnology

ER -

Compendium of 4,941 rumen metagenome-assembled genomes for rumen microbiome biology and enzyme discovery

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Understanding the functional and genomic architecture of the rumen microbiome affecting performance traits in bovines

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