Projects per year
Abstract / Description of output
Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.
Keywords / Materials (for Non-textual outputs)
- Journal Article
FingerprintDive into the research topics of 'Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry'. Together they form a unique fingerprint.
- 4 Finished
1/06/14 → 31/05/21