Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry

Adam Belsom, Gemma Mudd, Sven Giese, Manfred Auer, Juri Rappsilber

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.

Original languageEnglish
Pages (from-to)5319-5324
Number of pages6
JournalAnalytical Chemistry
Issue number10
Early online date21 Apr 2017
Publication statusPublished - 16 May 2017

Keywords / Materials (for Non-textual outputs)

  • Journal Article


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