Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

William H Wilson; Declan C Schroeder; Michael J Allen; Matthew T G Holden; Julian Parkhill; Bart G Barrell; Carol Churcher; Nancy Hamlin; Karen Mungall; Halina Norbertczak; Michael A Quail; Claire Price; Ester Rabbinowitsch; Danielle Walker; Marie Craigon; Douglas Roy; Peter Ghazal

doi:10.1126/science.1113109

Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

William H Wilson, Declan C Schroeder, Michael J Allen, Matthew T G Holden, Julian Parkhill, Bart G Barrell, Carol Churcher, Nancy Hamlin, Karen Mungall, Halina Norbertczak, Michael A Quail, Claire Price, Ester Rabbinowitsch, Danielle Walker, Marie Craigon, Douglas Roy, Peter Ghazal

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

Original language	English
Pages (from-to)	1090-2
Number of pages	3
Journal	Science
Volume	309
Issue number	5737
DOIs	https://doi.org/10.1126/science.1113109
Publication status	Published - 2005

Keywords / Materials (for Non-textual outputs)

Apoptosis
Base Composition
Ceramides
Computational Biology
DNA, Viral
DNA-Directed RNA Polymerases
Eukaryota
Gene Expression
Gene Expression Profiling
Genes, Viral
Genome, Viral
Oligonucleotide Array Sequence Analysis
Peptide Hydrolases
Phycodnaviridae
Promoter Regions, Genetic
Protein Subunits
Sequence Analysis, DNA
Sphingolipids
Transcription, Genetic
Virus Replication

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10.1126/science.1113109

Cite this

Wilson, W. H., Schroeder, D. C., Allen, M. J., Holden, M. T. G., Parkhill, J., Barrell, B. G., Churcher, C., Hamlin, N., Mungall, K., Norbertczak, H., Quail, M. A., Price, C., Rabbinowitsch, E., Walker, D., Craigon, M., Roy, D., & Ghazal, P. (2005). Complete genome sequence and lytic phase transcription profile of a Coccolithovirus. Science, 309(5737), 1090-2. https://doi.org/10.1126/science.1113109

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title = "Complete genome sequence and lytic phase transcription profile of a Coccolithovirus",

abstract = "The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.",

keywords = "Apoptosis, Base Composition, Ceramides, Computational Biology, DNA, Viral, DNA-Directed RNA Polymerases, Eukaryota, Gene Expression, Gene Expression Profiling, Genes, Viral, Genome, Viral, Oligonucleotide Array Sequence Analysis, Peptide Hydrolases, Phycodnaviridae, Promoter Regions, Genetic, Protein Subunits, Sequence Analysis, DNA, Sphingolipids, Transcription, Genetic, Virus Replication",

author = "Wilson, {William H} and Schroeder, {Declan C} and Allen, {Michael J} and Holden, {Matthew T G} and Julian Parkhill and Barrell, {Bart G} and Carol Churcher and Nancy Hamlin and Karen Mungall and Halina Norbertczak and Quail, {Michael A} and Claire Price and Ester Rabbinowitsch and Danielle Walker and Marie Craigon and Douglas Roy and Peter Ghazal",

year = "2005",

doi = "10.1126/science.1113109",

language = "English",

volume = "309",

pages = "1090--2",

journal = "Science",

publisher = "AMER ASSOC ADVANCEMENT SCIENCE",

number = "5737",

}

Wilson, WH, Schroeder, DC, Allen, MJ, Holden, MTG, Parkhill, J, Barrell, BG, Churcher, C, Hamlin, N, Mungall, K, Norbertczak, H, Quail, MA, Price, C, Rabbinowitsch, E, Walker, D, Craigon, M , Roy, D & Ghazal, P 2005, 'Complete genome sequence and lytic phase transcription profile of a Coccolithovirus', Science, vol. 309, no. 5737, pp. 1090-2. https://doi.org/10.1126/science.1113109

TY - JOUR

T1 - Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

AU - Wilson, William H

AU - Schroeder, Declan C

AU - Allen, Michael J

AU - Holden, Matthew T G

AU - Parkhill, Julian

AU - Barrell, Bart G

AU - Churcher, Carol

AU - Hamlin, Nancy

AU - Mungall, Karen

AU - Norbertczak, Halina

AU - Quail, Michael A

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Walker, Danielle

AU - Craigon, Marie

AU - Roy, Douglas

AU - Ghazal, Peter

PY - 2005

Y1 - 2005

N2 - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

AB - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

KW - Apoptosis

KW - Base Composition

KW - Ceramides

KW - Computational Biology

KW - DNA, Viral

KW - DNA-Directed RNA Polymerases

KW - Eukaryota

KW - Gene Expression

KW - Gene Expression Profiling

KW - Genes, Viral

KW - Genome, Viral

KW - Oligonucleotide Array Sequence Analysis

KW - Peptide Hydrolases

KW - Phycodnaviridae

KW - Promoter Regions, Genetic

KW - Protein Subunits

KW - Sequence Analysis, DNA

KW - Sphingolipids

KW - Transcription, Genetic

KW - Virus Replication

U2 - 10.1126/science.1113109

DO - 10.1126/science.1113109

M3 - Article

C2 - 16099989

VL - 309

SP - 1090

EP - 1092

JO - Science

JF - Science

IS - 5737

ER -

Complete genome sequence and lytic phase transcription profile of a Coccolithovirus

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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