Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

Kalya M. Hardwick , Gladys Bichang'a, Andnet Bayleyegn Abtew, Ryan Musumba Awori, Leah Cepko, Lorna Jemosop Chebon-Bore, Aistair Darby, Jon D. deVries, Jonathan Filee, Muna Fuad, Grace Gachara, Dedan Githae, Patrick Gunga, Madison Held, Hellen Kariuki, Evans Sioma Kataka, Susan Diana Kerfua, Kelvin Muteru Kimenyi, Bruno Le Ru, Ernest Mukweyi LutomiaElizabeth Ajema Luvai, Maureen K. Luvanda, Beatus Modest Lyimo, Eunice M. Machuka, Daniel G. Maeda, Solomon Maina, Peterson Gitonga Mathenge, Damaris Matoke-Muhia, Caitlin Miller, Silviane Aoko Miruka, Alfred Mitema, Antoinette Aluoch Miyunga, Sarah Akwabi Mukolwe, Edward Kirwa Muge, Simon Ngao Mule, Mary Kanyiri Murithi, Jean Pierre Musabyimana, Saphan Muzoora, Esther Wangui Mwangi, Harrison Ndung'u Mwangi, Nduta Mwangi, Ann Njeri Mwaura, Nelly Ndungu, Bidii Stephen Ngalah, Peter Njenga Ng'ang'a, Harun N. Njoroge, Brenda Nyaboke Nyandika, Johnson Ounya Nyasani, Faith Akinyi Obange, Shem Joabh Ochieng, Willingtone Otieno Odhiambo, Hellen Adhiambo Ogot, Awino Maureiq Edith Ojwang, Fred Odhiambo Osowo, Billy Omboki Ratemo, Tolbert Sonda , Alexander Ssamula, Sharon Towett-Kirui, Augustin Penda Twizerimana, Kelvin Mwangi Wachuin, Alex Paul Wacoo, Kevin Wamae, Mark Kilongosi Webale, Reuben Mangi Yaa, Dawn E. Gunderson-Rindal , Francesca Stomeo, Appolinaire Djikeng, Paul-Andre Calatayud, Sarah Schaack

Research output: Contribution to journalArticlepeer-review

Abstract

Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential nfectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.
Original languageEnglish
JournalGenome Biology and Evolution
DOIs
Publication statusPublished - 18 Sep 2020

Keywords

  • agricultural pest
  • Lepidoptera
  • insect genomics

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