Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

Kalya M.  Hardwick; Gladys Bichang'a; Andnet Bayleyegn Abtew; Ryan Musumba Awori; Leah Cepko; Lorna Jemosop Chebon-Bore; Aistair Darby; Jon D.  deVries; Jonathan Filee; Muna Fuad; Grace Gachara; Dedan Githae; Patrick Gunga; Madison Held; Hellen Kariuki; Evans Sioma Kataka; Susan Diana Kerfua; Kelvin Muteru Kimenyi; Bruno Le Ru; Ernest Mukweyi Lutomia; Elizabeth Ajema Luvai; Maureen K.  Luvanda; Beatus Modest  Lyimo; Eunice M.  Machuka; Daniel G.  Maeda; Solomon Maina; Peterson Gitonga Mathenge; Damaris Matoke-Muhia; Caitlin Miller; Silviane Aoko Miruka; Alfred Mitema; Antoinette Aluoch Miyunga; Sarah Akwabi Mukolwe; Edward Kirwa Muge; Simon Ngao Mule; Mary Kanyiri Murithi; Jean Pierre Musabyimana; Saphan Muzoora; Esther Wangui Mwangi; Harrison Ndung'u Mwangi; Nduta Mwangi; Ann Njeri Mwaura; Nelly Ndungu; Bidii Stephen Ngalah; Peter Njenga Ng'ang'a; Harun N.  Njoroge; Brenda Nyaboke Nyandika; Johnson Ounya Nyasani; Faith Akinyi Obange; Shem Joabh Ochieng; Willingtone Otieno Odhiambo; Hellen Adhiambo Ogot; Awino Maureiq Edith  Ojwang; Fred Odhiambo Osowo; Billy Omboki Ratemo; Tolbert Sonda; Alexander Ssamula; Sharon  Towett-Kirui; Augustin Penda Twizerimana; Kelvin Mwangi Wachuin; Alex Paul Wacoo; Kevin Wamae; Mark Kilongosi  Webale; Reuben Mangi Yaa; Dawn E.  Gunderson-Rindal; Francesca Stomeo; Appolinaire Djikeng; Paul-Andre Calatayud; Sarah Schaack

doi:10.1093/gbe/evaa195

Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

Kalya M. Hardwick , Gladys Bichang'a, Andnet Bayleyegn Abtew, Ryan Musumba Awori, Leah Cepko, Lorna Jemosop Chebon-Bore, Aistair Darby, Jon D. deVries, Jonathan Filee, Muna Fuad, Grace Gachara, Dedan Githae, Patrick Gunga, Madison Held, Hellen Kariuki, Evans Sioma Kataka, Susan Diana Kerfua, Kelvin Muteru Kimenyi, Bruno Le Ru, Ernest Mukweyi LutomiaElizabeth Ajema Luvai, Maureen K. Luvanda, Beatus Modest Lyimo, Eunice M. Machuka, Daniel G. Maeda, Solomon Maina, Peterson Gitonga Mathenge, Damaris Matoke-Muhia, Caitlin Miller, Silviane Aoko Miruka, Alfred Mitema, Antoinette Aluoch Miyunga, Sarah Akwabi Mukolwe, Edward Kirwa Muge, Simon Ngao Mule, Mary Kanyiri Murithi, Jean Pierre Musabyimana, Saphan Muzoora, Esther Wangui Mwangi, Harrison Ndung'u Mwangi, Nduta Mwangi, Ann Njeri Mwaura, Nelly Ndungu, Bidii Stephen Ngalah, Peter Njenga Ng'ang'a, Harun N. Njoroge, Brenda Nyaboke Nyandika, Johnson Ounya Nyasani, Faith Akinyi Obange, Shem Joabh Ochieng, Willingtone Otieno Odhiambo, Hellen Adhiambo Ogot, Awino Maureiq Edith Ojwang, Fred Odhiambo Osowo, Billy Omboki Ratemo, Tolbert Sonda , Alexander Ssamula, Sharon Towett-Kirui, Augustin Penda Twizerimana, Kelvin Mwangi Wachuin, Alex Paul Wacoo, Kevin Wamae, Mark Kilongosi Webale, Reuben Mangi Yaa, Dawn E. Gunderson-Rindal , Francesca Stomeo, Appolinaire Djikeng, Paul-Andre Calatayud, Sarah Schaack

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential nfectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.

Original language	English
Journal	Genome Biology and Evolution
DOIs	https://doi.org/10.1093/gbe/evaa195
Publication status	Published - 18 Sept 2020

Keywords / Materials (for Non-textual outputs)

agricultural pest
Lepidoptera
insect genomics

Access to Document

10.1093/gbe/evaa195

evaa195Final published version, 404 KBLicence: Creative Commons: Attribution Non-Commercial (CC-BY-NC)

Cite this

Hardwick , K. M., Bichang'a, G., Abtew, A. B., Awori, R. M., Cepko, L., Chebon-Bore, L. J., Darby, A., deVries, J. D., Filee, J., Fuad, M., Gachara, G., Githae, D., Gunga, P., Held, M., Kariuki, H., Sioma Kataka, E., Kerfua, S. D., Kimenyi, K. M., Le Ru, B., ... Schaack, S. (2020). Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures. Genome Biology and Evolution. https://doi.org/10.1093/gbe/evaa195

@article{6cc4b524dae64287a7363f716fc5fe7f,

title = "Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures",

abstract = "Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential nfectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.",

keywords = "agricultural pest, Lepidoptera, insect genomics",

author = "Hardwick, {Kalya M.} and Gladys Bichang'a and Abtew, {Andnet Bayleyegn} and Awori, {Ryan Musumba} and Leah Cepko and Chebon-Bore, {Lorna Jemosop} and Aistair Darby and deVries, {Jon D.} and Jonathan Filee and Muna Fuad and Grace Gachara and Dedan Githae and Patrick Gunga and Madison Held and Hellen Kariuki and {Sioma Kataka}, Evans and Kerfua, {Susan Diana} and Kimenyi, {Kelvin Muteru} and {Le Ru}, Bruno and Lutomia, {Ernest Mukweyi} and Luvai, {Elizabeth Ajema} and Luvanda, {Maureen K.} and Lyimo, {Beatus Modest} and Machuka, {Eunice M.} and Maeda, {Daniel G.} and Solomon Maina and Mathenge, {Peterson Gitonga} and Damaris Matoke-Muhia and Caitlin Miller and Miruka, {Silviane Aoko} and Alfred Mitema and Miyunga, {Antoinette Aluoch} and Mukolwe, {Sarah Akwabi} and Muge, {Edward Kirwa} and Mule, {Simon Ngao} and Murithi, {Mary Kanyiri} and Musabyimana, {Jean Pierre} and Saphan Muzoora and Mwangi, {Esther Wangui} and Mwangi, {Harrison Ndung'u} and Nduta Mwangi and Mwaura, {Ann Njeri} and Nelly Ndungu and Ngalah, {Bidii Stephen} and Ng'ang'a, {Peter Njenga} and Njoroge, {Harun N.} and Nyandika, {Brenda Nyaboke} and Nyasani, {Johnson Ounya} and Obange, {Faith Akinyi} and Ochieng, {Shem Joabh} and Odhiambo, {Willingtone Otieno} and Ogot, {Hellen Adhiambo} and Ojwang, {Awino Maureiq Edith} and Osowo, {Fred Odhiambo} and Ratemo, {Billy Omboki} and Tolbert Sonda and Alexander Ssamula and Sharon Towett-Kirui and Twizerimana, {Augustin Penda} and Wachuin, {Kelvin Mwangi} and Wacoo, {Alex Paul} and Kevin Wamae and Webale, {Mark Kilongosi} and Yaa, {Reuben Mangi} and Gunderson-Rindal, {Dawn E.} and Francesca Stomeo and Appolinaire Djikeng and Paul-Andre Calatayud and Sarah Schaack",

year = "2020",

month = sep,

day = "18",

doi = "10.1093/gbe/evaa195",

language = "English",

journal = "Genome Biology and Evolution",

issn = "1759-6653",

publisher = "Oxford University Press",

}

Hardwick , KM, Bichang'a, G, Abtew, AB, Awori, RM, Cepko, L, Chebon-Bore, LJ, Darby, A, deVries, JD, Filee, J, Fuad, M, Gachara, G, Githae, D, Gunga, P, Held, M, Kariuki, H, Sioma Kataka, E, Kerfua, SD, Kimenyi, KM, Le Ru, B, Lutomia, EM, Luvai, EA, Luvanda, MK, Lyimo, BM, Machuka, EM, Maeda, DG, Maina, S, Mathenge, PG, Matoke-Muhia, D, Miller, C, Miruka, SA, Mitema, A, Miyunga, AA, Mukolwe, SA, Muge, EK, Mule, SN, Murithi, MK, Musabyimana, JP, Muzoora, S, Mwangi, EW, Mwangi, HN, Mwangi, N, Mwaura, AN, Ndungu, N, Ngalah, BS, Ng'ang'a, PN, Njoroge, HN, Nyandika, BN, Nyasani, JO, Obange, FA, Ochieng, SJ, Odhiambo, WO, Ogot, HA, Ojwang, AME, Osowo, FO, Ratemo, BO, Sonda , T, Ssamula, A, Towett-Kirui, S, Twizerimana, AP, Wachuin, KM, Wacoo, AP, Wamae, K, Webale, MK, Yaa, RM, Gunderson-Rindal , DE, Stomeo, F, Djikeng, A, Calatayud, P-A & Schaack, S 2020, 'Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures', Genome Biology and Evolution. https://doi.org/10.1093/gbe/evaa195

TY - JOUR

T1 - Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

AU - Hardwick , Kalya M.

AU - Bichang'a, Gladys

AU - Abtew, Andnet Bayleyegn

AU - Awori, Ryan Musumba

AU - Cepko, Leah

AU - Chebon-Bore, Lorna Jemosop

AU - Darby, Aistair

AU - deVries, Jon D.

AU - Filee, Jonathan

AU - Fuad, Muna

AU - Gachara, Grace

AU - Githae, Dedan

AU - Gunga, Patrick

AU - Held, Madison

AU - Kariuki, Hellen

AU - Sioma Kataka, Evans

AU - Kerfua, Susan Diana

AU - Kimenyi, Kelvin Muteru

AU - Le Ru, Bruno

AU - Lutomia, Ernest Mukweyi

AU - Luvai, Elizabeth Ajema

AU - Luvanda, Maureen K.

AU - Lyimo, Beatus Modest

AU - Machuka, Eunice M.

AU - Maeda, Daniel G.

AU - Maina, Solomon

AU - Mathenge, Peterson Gitonga

AU - Matoke-Muhia, Damaris

AU - Miller, Caitlin

AU - Miruka, Silviane Aoko

AU - Mitema, Alfred

AU - Miyunga, Antoinette Aluoch

AU - Mukolwe, Sarah Akwabi

AU - Muge, Edward Kirwa

AU - Mule, Simon Ngao

AU - Murithi, Mary Kanyiri

AU - Musabyimana, Jean Pierre

AU - Muzoora, Saphan

AU - Mwangi, Esther Wangui

AU - Mwangi, Harrison Ndung'u

AU - Mwangi, Nduta

AU - Mwaura, Ann Njeri

AU - Ndungu, Nelly

AU - Ngalah, Bidii Stephen

AU - Ng'ang'a, Peter Njenga

AU - Njoroge, Harun N.

AU - Nyandika, Brenda Nyaboke

AU - Nyasani, Johnson Ounya

AU - Obange, Faith Akinyi

AU - Ochieng, Shem Joabh

AU - Odhiambo, Willingtone Otieno

AU - Ogot, Hellen Adhiambo

AU - Ojwang, Awino Maureiq Edith

AU - Osowo, Fred Odhiambo

AU - Ratemo, Billy Omboki

AU - Sonda , Tolbert

AU - Ssamula, Alexander

AU - Towett-Kirui, Sharon

AU - Twizerimana, Augustin Penda

AU - Wachuin, Kelvin Mwangi

AU - Wacoo, Alex Paul

AU - Wamae, Kevin

AU - Webale, Mark Kilongosi

AU - Yaa, Reuben Mangi

AU - Gunderson-Rindal , Dawn E.

AU - Stomeo, Francesca

AU - Djikeng, Appolinaire

AU - Calatayud, Paul-Andre

AU - Schaack, Sarah

PY - 2020/9/18

Y1 - 2020/9/18

N2 - Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential nfectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.

AB - Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential nfectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.

KW - agricultural pest

KW - Lepidoptera

KW - insect genomics

U2 - 10.1093/gbe/evaa195

DO - 10.1093/gbe/evaa195

M3 - Article

SN - 1759-6653

JO - Genome Biology and Evolution

JF - Genome Biology and Evolution

ER -

Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Cite this