Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe

David Girdwood, Morag Robertson, Colin Gordon

Research output: Contribution to journalArticlepeer-review

Abstract

In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.
Original languageEnglish
Pages (from-to)1522-8
Number of pages7
JournalFEBS Letters
Volume586
Issue number10
DOIs
Publication statusPublished - 21 May 2012

Keywords

  • Base Sequence
  • Cullin Proteins
  • DNA Primers
  • Ligases
  • Molecular Sequence Data
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins

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