Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe

David Girdwood; Morag Robertson; Colin Gordon

doi:10.1016/j.febslet.2012.04.011

Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe

David Girdwood, Morag Robertson, Colin Gordon

MRC Human Genetics Unit

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.

Original language	English
Pages (from-to)	1522-8
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2012.04.011
Publication status	Published - 21 May 2012

Keywords / Materials (for Non-textual outputs)

Base Sequence
Cullin Proteins
DNA Primers
Ligases
Molecular Sequence Data
Schizosaccharomyces
Schizosaccharomyces pombe Proteins

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10.1016/j.febslet.2012.04.011

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title = "Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe",

abstract = "In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.",

keywords = "Base Sequence, Cullin Proteins, DNA Primers, Ligases, Molecular Sequence Data, Schizosaccharomyces, Schizosaccharomyces pombe Proteins",

author = "David Girdwood and Morag Robertson and Colin Gordon",

year = "2012",

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day = "21",

doi = "10.1016/j.febslet.2012.04.011",

language = "English",

volume = "586",

pages = "1522--8",

journal = "FEBS Letters",

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T1 - Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe

AU - Girdwood, David

AU - Robertson, Morag

AU - Gordon, Colin

PY - 2012/5/21

Y1 - 2012/5/21

N2 - In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.

AB - In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.

KW - Base Sequence

KW - Cullin Proteins

KW - DNA Primers

KW - Ligases

KW - Molecular Sequence Data

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

U2 - 10.1016/j.febslet.2012.04.011

DO - 10.1016/j.febslet.2012.04.011

M3 - Article

C2 - 22673520

SN - 0014-5793

VL - 586

SP - 1522

EP - 1528

JO - FEBS Letters

JF - FEBS Letters

IS - 10

ER -

Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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