Contributions of polarizing region cells to digit pattern

M. Towers, J. Signolet, H. Downie, I. Delgado, A. Sherman, H. Sang, C. Tickle

Research output: Contribution to journalMeeting abstract


In the classical model derived from experiments in the chick wing, the antero-posterior (thumb-little finger) pattern of digits arises in response to a concentration gradient of Sonic hedgehog (Shh) protein produced by the polarizing region - a specialized group of cells located at the posterior margin of the early limb bud. In the mouse, however, it has been demonstrated that the two most posterior of the five digits are derived entirely from the polarizing region by proliferation without the requirement of Shh diffusion. To understand the contribution and significance that polarizing region cell proliferation has in forming different digit patterns, we grafted polarizing regions from a transgenic chicken line stably expressing Green Fluorescent Protein (GFP) into normal wing buds (three digits), or leg buds (four digits). We will describe how these fate-maps behave when either Shh signalling is dampened, or proliferation is inhibited. We will present a model outlining our findings, highlighting the evolutionary implications.
Original languageUndefined/Unknown
Pages (from-to)S211-S212, abstract 12-P056
JournalMechanisms of Development
Issue numberSuppt. 1
Publication statusPublished - 2009
Event16th International Society of Developmental Biologists Congress 2009 - Edinburgh, United Kingdom
Duration: 6 Sep 200910 Sep 2009

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