Control of expression of the I factor, a LINE-like transposable element in Drosophila melanogaster

A Udomkit, S Forbes, C McLean, I Arkhipova, D J Finnegan

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

I factors are LINE-like transposable elements in the genome of Drosophila melanogaster. They normally transpose infrequently but are activated in the germline of female progeny of crosses between males of a strain that contains complete elements, an I or inducer strain and females of a strain that does not, an R or reactive strain. This causes a phenomenon known as I-R hybrid dysgenesis, We have previously shown that the I factor promoter lies between nucleotides 1 and 30. Here we demonstrate that expression of this promoter is regulated by nucleotides 41-186 of the I factor. This sequence can act as an enhancer as it stimulates expression of the hsp70 promoter in ovaries in the absence of heat-shock. Within this region there is a site that is required for promoter activity and that is recognized by a sequence-specific binding protein, We propose that this protein contributes to the enhancer activity of nucleotides 41-186 and that reduced I factor expression in inducer strains is due to titration of this protein or others that interact with it.

Original languageEnglish
Pages (from-to)3174-3181
Number of pages8
JournalEMBO Journal
Volume15
Issue number12
Publication statusPublished - 17 Jun 1996

Keywords / Materials (for Non-textual outputs)

  • Drosophila
  • I factor
  • transposable element

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