COP1 contributes to UVB-induced signaling in human keratinocytes

Agnes Kinyó, Zsuzsanna Kiss-László, Szabolcs Hambalkó, Attila Bebes, Mária Kiss, Márta Széll, Zsuzsanna Bata-Csörgo, Ferenc Nagy, Lajos Kemény

Research output: Contribution to journalArticlepeer-review

Abstract

UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.
Original languageEnglish
Pages (from-to)541-5
Number of pages5
JournalJournal of Investigative Dermatology
Volume130
Issue number2
DOIs
Publication statusPublished - Feb 2010

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