Correlation between three assay systems for anti-Mullerian hormone (AMH) determination

Hang Wun Raymond Li, Ernest Hung Yu Ng, Benancy Po Chau Wong, Richard A. Anderson, Pak Chung Ho, William Shu Biu Yeung

Research output: Contribution to journalArticlepeer-review

Abstract

Analysis of anti-Mullerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL).

A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L).

We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R-2 = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 x AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 x AMH (DSL) - 1.270 respectively.

AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended.

Original languageEnglish
Pages (from-to)1443-1446
Number of pages4
JournalJournal of Assisted Reproduction and Genetics
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • ASSISTED REPRODUCTION
  • Anti-Mullerian hormone
  • DSL ELISA
  • LIVE BIRTH
  • SERUM ANTIMULLERIAN HORMONE
  • BECKMAN COULTER ELISA
  • PREDICTION
  • ELISA kit
  • MENSTRUAL-CYCLE

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