Correlation of EPHX1, GSTP1, GSTM1, and GSTT1 genetic polymorphisms with antioxidative stress markers in chronic obstructive pulmonary disease

Ramzi Lakhdar*, Sabri Denden, Manel Haj Mouhamed, Abdelkader Chalgoum, Nadia Leban, Jalel Knani, Gerard Lefranc, Abelhadi Miled, Jemni Ben Chibani, Amel Haj Khelil

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study was undertaken to ascertain if a relationship existed between oxidative status and polymorphisms of microsomal epoxide hydrolase X1 (EPHX1), glutathione S-transferase P1 (GSTP1), GSTM1, and GSTT1 in chronic obstructive pulmonary disease (COPD). Erythrocyte glutathione peroxidase (GSH-px), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and plasma GST activities and total antioxidant status (TAS) as antioxidative stress markers were determined and compared either with individual and combined genotypes of EPHX1 exon 3, GSTP1 exon 5, GSTM1, and GSTT1 polymorphisms in COPD patients and healthy controls from the central area of Tunisia. Statistical data processing revealed significantly lower GSH-px, GR, SOD, CAT, GST, and TAS values in COPD patients in comparison to the control group (P <.001). As for genotypes, there was a no significant association in each of the 6 parameters and individual genotypes (P > .05). A significant correlation between the studied parameters and combined null GSTM1/null GSTT1 (GSH-px: P <.001, GR: P = .026, CAT: P = .018, GST: P = .022, TAS: P = .046), His113His EPHX1/null GSTM1 (GSH-px: P = .001, GST: P = .0012, TAS: P = .013), His113His EPHX1/Val105Val GSTP1 (GSH-px: P = .048, CAT: P = .026, GST: P = .031), and null GSTM1/Val105Val GSTP1 (GSH-px: P = .011, GR: P = .0028, GST: P = .0054, TAS: P = .032) was found in patients. In conclusion, combined genetic polymorphisms of GSTM1, GSTT1, GSTP1, and EPHX1 may have favorable effects on redox balance in COPD patients.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalExperimental Lung Research
Volume37
Issue number4
DOIs
Publication statusPublished - May 2011

Keywords

  • COPD
  • genetic polymorphism
  • glutathione S-transferase
  • microsomal epoxide hydrolase
  • oxidative stress
  • GLUTATHIONE S-TRANSFERASES
  • MICROSOMAL EPOXIDE HYDROLASE
  • OXIDATIVE STRESS
  • SUPEROXIDE-DISMUTASE
  • FUNCTIONAL-ACTIVITY
  • LUNG-FUNCTION
  • SMOKERS
  • SMOKING
  • PLASMA

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