Correlation of Regulatory T cells with resistance and susceptibility to Teladorsagia circumcincta infection in lambs

Virginia M Venturina, Anton Gossner, David Taylor, John Hopkins

Research output: Contribution to conferencePoster

Abstract / Description of output

Teladorsagia circumcincta is a common and economically important abomasal nematode of sheep, which shows genetically-linked heterogeneity to infection. Female lambs from parents with variability in resistance to T. circumcincta were trickle infected with L3 larvae for 3 months. A range of parameters were measured throughout the period and at post-mortem (13 weeks), which resulted in the identification of 57 lambs with a range of susceptibilities to infection. Faecal egg count was positively correlated with worm fecundity and worm length and inversely correlated with IgA anti-T. circumcincta antibody levels and body weight (Beraldi et al. 2008. Int. J. Parasitol. 38; 1567-77).

This project is investigating the role of the immune response in T. circumcincta resistance. T cell subsets are being quantified by immunohistology including the visualization of Treg cells by anti-Foxp3/CD25; and we have cloned and sequenced transcripts for a range of sheep regulatory cytokines associated with TH1, TH2, TH17 and Treg functions; including IFN, TGFβ, IL-4, IL-10, IL-17E, IL-21, IL-23A, and IL-35 (EBI3). qRT-PCR assays have been developed for each of these sheep cytokines, and for sheep Foxp3, to quantify expression levels in the abomasal mucosae and gastric lymph node. Analyses of these data will be presented and correlated with relevant phenotypic parameters to help clarify the immunological basis for variation in susceptibility to T. circumcincta infection.
Original languageEnglish
Publication statusPublished - 30 Mar 2010
EventBritish Society of parasitology Spring Meeting - Nottingham, United Kingdom
Duration: 30 Mar 20102 Apr 2010

Conference

ConferenceBritish Society of parasitology Spring Meeting
Country/TerritoryUnited Kingdom
CityNottingham
Period30/03/102/04/10

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