Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion

Shannon J L Pinnington, John F Marshall, Ann P Wheeler

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively.

Original languageEnglish
Title of host publicationCorrelative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasio
Pages253-265
Number of pages13
Volume1764
DOIs
Publication statusPublished - 1 Apr 2018

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Carcinoma, Squamous Cell/pathology
  • Humans
  • Imaging, Three-Dimensional/methods
  • Lighting/instrumentation
  • Microscopy, Fluorescence/methods
  • Mouth Neoplasms/pathology
  • Neoplasm Invasiveness
  • Podosomes/pathology
  • Single Molecule Imaging/methods
  • Tumor Cells, Cultured

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