Abstract
Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively.
Original language | English |
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Title of host publication | Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasio |
Pages | 253-265 |
Number of pages | 13 |
Volume | 1764 |
DOIs | |
Publication status | Published - 1 Apr 2018 |
Publication series
Name | Methods in molecular biology (Clifton, N.J.) |
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Publisher | Humana Press |
ISSN (Print) | 1064-3745 |
Keywords / Materials (for Non-textual outputs)
- Carcinoma, Squamous Cell/pathology
- Humans
- Imaging, Three-Dimensional/methods
- Lighting/instrumentation
- Microscopy, Fluorescence/methods
- Mouth Neoplasms/pathology
- Neoplasm Invasiveness
- Podosomes/pathology
- Single Molecule Imaging/methods
- Tumor Cells, Cultured
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Ann Wheeler
- Deanery of Molecular, Genetic and Population Health Sciences - Head of Advanced BioImaging Facility
Person: Academic: Research Active