Corticotropin-releasing factor (CRF) gene family in the brain of the teleost fish Catostomus commersoni (white sucker): molecular analysis predicts distinct precursors for two CRFs and one urotensin I peptide

S D Morley, C Schönrock, D Richter, Y Okawara, K Lederis

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular cloning experiments indicate the presence of two distinct CRF genes in the sucker genome encoding independent 162-amino-acid precursors, which both consist of a signal sequence, succeeded by a cryptic peptide and subsequently by the hormone moiety. The two 41-amino-acid CRF peptides differ by an Ala-->Val substitution at amino acid position 28. CRF transcripts are primarily found in the sucker pre-optic nucleus (PON), to a much lesser extent in the lateral tuberal nucleus (LTN). In contrast, urotensin I (U I) encoding mRNA is equally present in both tissues. In urophysectomized fish, U I mRNA is elevated especially in LTN tissue, while CRF mRNA levels remain more or less constant in the PON and LTN regions.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalMolecular Marine Biology and Biotechnology
Volume1
Issue number1
Publication statusPublished - Sep 1991

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Brain
  • Cloning, Molecular
  • Corticotropin-Releasing Hormone
  • Fishes
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Multigene Family
  • Poly A
  • Preoptic Area
  • Protein Precursors
  • RNA
  • RNA, Messenger
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Urotensins

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