Cotinine Exposure Increases Fallopian Tube PROKR1 Expression via Nicotinic AChR alpha-7: A Potential Mechanism Explaining the Link between Smoking and Tubal Ectopic Pregnancy

Julie L. V. Shaw, Elizabeth Oliver, Kai-Fai Lee, Gary Entrican, Henry N. Jabbour, Hilary O. D. Critchley, Andrew W. Horne

Research output: Contribution to journalArticlepeer-review

Abstract

Tubal ectopic pregnancy (EP) is the most common cause of maternal mortality in the first trimester of pregnancy; however, its etiology is uncertain. In EP, embryo retention within the Fallopian tube (FT) is thought to be due to impaired smooth muscle contractility (SMC) and alterations in the tubal microenvironment. Smoking is a major risk factor for EP. FTs from women with EP exhibit altered prokineticin receptor-1 (PROKR1) expression, the receptor for prokineticins (PROK). PROK1 is angiogenic, regulates SMC, and is involved in intrauterine implantation. We hypothesized that smoking predisposes women to EP by altering tubal PROKR1 expression. Sera/FT were collected at hysterectomy (n=21). Serum levels of the smoking metabolite, cotinine, were measured by enzyme-linked immunosorbent assay. FTs were analyzed by q-RT-PCR, immunohistochemistry, and Western blotting for expression of PROKR1 and the predicted cotinine receptor, nicotinic acetylcholine receptor α-7 (AChRα-7). FT explants (n=4) and oviductal epithelial cells (cell line OE-E6/E7) were treated with cotinine and an nAChRα-7 antagonist. PROKR1 transcription was higher in FTs from smokers (P
Original languageEnglish
Pages (from-to)2509-2515
Number of pages7
JournalAmerican Journal of Pathology
Volume177
Issue number5
DOIs
Publication statusPublished - Nov 2010

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