Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer

P Cramer, J F Cáceres, D Cazalla, S Kadener, A F Muro, F E Baralle, A R Kornblihtt

Research output: Contribution to journalArticlepeer-review

Abstract

Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE.
Original languageEnglish
Pages (from-to)251-8
Number of pages8
JournalMolecular Cell
Volume4
Issue number2
Publication statusPublished - Aug 1999

Keywords

  • Alternative Splicing
  • Base Sequence
  • Enhancer Elements, Genetic
  • Exons
  • Fibronectins
  • Globins
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Promoter Regions, Genetic
  • RNA Polymerase II
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic
  • Transfection
  • Tumor Cells, Cultured
  • beta-Galactosidase

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