Abstract
Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐tagged proteins on living cells was derived.
Original language | English |
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Pages (from-to) | 1391-1395 |
Number of pages | 5 |
Journal | ChemBioChem |
Volume | 9 |
Issue number | 9 |
DOIs | |
Publication status | Published - 9 Jun 2008 |