Covalent fluorescence labeling of His-tagged proteins on the surface of living cells

Martin Hintersteiner, Thomas Weidemann, Thierry Kimmerlin, Nimet Filiz, Christof Buehler, Manfred Auer

Research output: Contribution to journalArticlepeer-review

Abstract

Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐tagged proteins on living cells was derived.
Original languageEnglish
Pages (from-to)1391-1395
Number of pages5
JournalChemBioChem
Volume9
Issue number9
DOIs
Publication statusPublished - 9 Jun 2008

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