Abstract / Description of output
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.
Original language | English |
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Pages (from-to) | 549-551 |
Number of pages | 3 |
Journal | Nature Biotechnology |
Volume | 26 |
Issue number | 5 |
DOIs | |
Publication status | Published - Apr 2008 |
Keywords / Materials (for Non-textual outputs)
- Animals CpG Islands/*genetics Gene Targeting/*methods Gene Therapy/*methods Inflammation/*genetics/*prevention & control Lung/*metabolism Plasmids/*genetics/*therapeutic use