Creating resistance to avian influenza infection through genome editing of the ANP32 gene family

Alewo Idoko-Akoh*, Daniel H. Goldhill, Carol M. Sheppard, Dagmara Bialy, Jessica L. Quantrill, Ksenia Sukhova, Jonathan C. Brown, Samuel Richardson, Ciara Campbell, Lorna Taylor, Adrian Sherman, Salik Nazki, Jason S. Long, Michael A Skinner, Holly Shelton, Helen Sang*, Wendy S. Barclay*, Mike McGrew

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Chickens genetically resistant to avian influenza could prevent future outbreaks. In chickens, influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions that prevent viral polymerase interaction. After IAV challenge, 9/10 edited chickens remain uninfected. Challenge with a higher dose, however, led to breakthrough infections. Breakthrough IAV virus contained IAV polymerase gene mutations that conferred adaptation to the edited chicken ANP32A. Unexpectedly, this virus also replicated in chicken embryos edited to remove the entire ANP32A gene and instead co-opted alternative ANP32 protein family members, chicken ANP32B and ANP32E. Additional genome editing for removal of ANP32B and ANP32E eliminated all viral growth in chicken cells. Our data illustrate a first proof of concept step to generate IAV-resistant chickens and show that multiple genetic modifications will be required to curtail viral escape.

Original languageEnglish
Article number6136
Pages (from-to)1-15
Number of pages15
JournalNature Communications
Issue number945
Early online date10 Oct 2023
Publication statusPublished - Dec 2023

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Chick Embryo
  • Chickens/genetics
  • Gene Editing
  • Influenza A virus/genetics
  • Influenza in Birds/genetics
  • RNA-Dependent RNA Polymerase/genetics


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