CRISPR-cas system: biological function in microbes and its use to treat antimicrobial resistant pathogens

Muhammad Abu Bakr Shabbir; Muhammad Zubair Shabbir; Qin Wu; Sammina Mahmood; Abdul Sajid; Muhammad Kashif Maan; Saeed Ahmed; Umer Naveed; Haihong Hao; Zonghui Yuan

doi:10.1186/s12941-019-0317-x

CRISPR-cas system: biological function in microbes and its use to treat antimicrobial resistant pathogens

Muhammad Abu Bakr Shabbir, Muhammad Zubair Shabbir, Qin Wu, Sammina Mahmood, Abdul Sajid, Muhammad Kashif Maan, Saeed Ahmed, Umer Naveed, Haihong Hao, Zonghui Yuan

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Review article › peer-review

Abstract / Description of output

The development of antibiotic resistance in bacteria is a major public health threat. Infection rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led to development of different strategies to combat the antimicrobial resistance. In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene. However, there is still challenge to practice these methods in field against emerging antimicrobial resistant pathogens.

Original language	English
Pages (from-to)	21
Journal	Annals of Clinical Microbiology and Antimicrobials
Volume	18
Issue number	1
DOIs	https://doi.org/10.1186/s12941-019-0317-x
Publication status	Published - 5 Jul 2019

Keywords / Materials (for Non-textual outputs)

CRISPR-cas system
Antimicrobial resistance
Cas9
Antibacterials
Intracellular infection

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10.1186/s12941-019-0317-x

CRISPR-cas system
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Cite this

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title = "CRISPR-cas system: biological function in microbes and its use to treat antimicrobial resistant pathogens",

abstract = "The development of antibiotic resistance in bacteria is a major public health threat. Infection rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led to development of different strategies to combat the antimicrobial resistance. In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene. However, there is still challenge to practice these methods in field against emerging antimicrobial resistant pathogens.",

keywords = "CRISPR-cas system, Antimicrobial resistance, Cas9, Antibacterials, Intracellular infection",

author = "Shabbir, {Muhammad Abu Bakr} and Shabbir, {Muhammad Zubair} and Qin Wu and Sammina Mahmood and Abdul Sajid and Maan, {Muhammad Kashif} and Saeed Ahmed and Umer Naveed and Haihong Hao and Zonghui Yuan",

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doi = "10.1186/s12941-019-0317-x",

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TY - JOUR

T1 - CRISPR-cas system

T2 - biological function in microbes and its use to treat antimicrobial resistant pathogens

AU - Shabbir, Muhammad Abu Bakr

AU - Shabbir, Muhammad Zubair

AU - Wu, Qin

AU - Mahmood, Sammina

AU - Sajid, Abdul

AU - Maan, Muhammad Kashif

AU - Ahmed, Saeed

AU - Naveed, Umer

AU - Hao, Haihong

AU - Yuan, Zonghui

PY - 2019/7/5

Y1 - 2019/7/5

N2 - The development of antibiotic resistance in bacteria is a major public health threat. Infection rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led to development of different strategies to combat the antimicrobial resistance. In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene. However, there is still challenge to practice these methods in field against emerging antimicrobial resistant pathogens.

AB - The development of antibiotic resistance in bacteria is a major public health threat. Infection rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led to development of different strategies to combat the antimicrobial resistance. In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene. However, there is still challenge to practice these methods in field against emerging antimicrobial resistant pathogens.

KW - CRISPR-cas system

KW - Antimicrobial resistance

KW - Cas9

KW - Antibacterials

KW - Intracellular infection

U2 - 10.1186/s12941-019-0317-x

DO - 10.1186/s12941-019-0317-x

M3 - Review article

C2 - 31277669

SN - 1476-0711

VL - 18

SP - 21

JO - Annals of Clinical Microbiology and Antimicrobials

JF - Annals of Clinical Microbiology and Antimicrobials

IS - 1

ER -

CRISPR-cas system: biological function in microbes and its use to treat antimicrobial resistant pathogens

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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