Original language | English |
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Journal | Alzheimer's & Dementia: The Journal of the Alzheimer's Association |
Early online date | 22 Aug 2022 |
DOIs | |
Publication status | E-pub ahead of print - 22 Aug 2022 |
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In: Alzheimer's & Dementia: The Journal of the Alzheimer's Association, 22.08.2022.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - CRISPR/Cas9 screen in human iPSC‐derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity
AU - Guo, Wenting
AU - Wang, Haibo
AU - Kumar Tharkeshwar, Arun
AU - Couthouis, Julien
AU - Braems, Elke
AU - Masrori, Pegah
AU - Van Schoor, Evelien
AU - Fan, Yannan
AU - Ahuja, Karan
AU - Moisse, Matthieu
AU - Jacquemyn, Maarten
AU - Furtado Madeiro Da Costa, Rodrigo
AU - Gajjar, Madhavsai
AU - Balusu, Sriram
AU - Tricot, Tine
AU - Fumagalli, Laura
AU - Hersmus, Nicole
AU - Janky, Rekin's
AU - Impens, Francis
AU - Vanden Berghe, Pieter
AU - Ho, Ritchie
AU - Thal, Dietmar Rudolf
AU - Vandenberghe, Rik
AU - Hegde, Muralidhar L.
AU - Chandran, Siddharthan
AU - De Strooper, Bart
AU - Daelemans, Dirk
AU - Van Damme, Philip
AU - Van Den Bosch, Ludo
AU - Verfaillie, Catherine
N1 - Funding Information: We thank Dr. Steven Boeynaems and Prof. Aaron D. Gitler from Stanford University for helping with the international collaboration and for discussion of the screen analysis. We thank Dr. Stefaan Derveaux and the members from the VIB Nucleomics Core for helping with the RNA seq and NGS. We thank Ms. Delphi Van Haver and the members from the VIB Proteomics Core for helping with the proteomics experiments. We thank Ms. Kristien Minner for helping with the sgRNA lentiviral vector production. We thank Dr. Arpan R. Mehta and Prof. Bhuvaneish T. Selvaraj from Edinburgh University for helping with the mechanistic exploration. We thank Mr. Niels Vidal and Ms. Annet Storm for helping with the real‐time PCR. W.G. holds a postdoctoral mandate fellowship from KU Leuven (PDM/18/213). E.B. holds a PhD fellowship from FWO (1145621N). E.V.S. holds a PhD fellowship from FWO (1S46219N). K.A. holds a PhD fellowship from FWO (1S03422N). S.B. holds a postdoctoral fellowship from FWO (12P5919N). T.T. holds a PhD fellowship from FWO (1185920N). This work was supported by an Opening the Future grant to C.V., L.V.D.B., and B.D.S.; and IWT‐ 150031‐iPSCAF grant to C.V., L.V.D.B., and P.V.D.; C.V. also received support from KU Leuven C1 grant (C14/17/111 ‐3D‐MuSYC) and an FWO‐SBO‐OrganID‐ S001221N grant. This work was also supported by the KU Leuven C1 (C14‐17‐107 [to L.V.D.B., P.V.D., D.R.T.]), the Fund for Scientific Research Flanders (FWO, 1520019N to W.G., G0F8516N to D.R.T.), ALS Liga Belgium (A Cure for ALS), and the European Community's Health Seventh Framework Programme (259867).A. K. T holds a 'Stichting Alzheimer Onderzoek' grant (SAO‐FRA 2020/0002 pilot grant). P.V.D. holds a senior clinical investigatorship of the FWO and is supported by the E. von Behring Chair for Neuromuscular and Neurodegenerative Disorders and the KU Leuven funds “Een Hart voor ALS,” “Laeversfonds voor ALS Onderzoek” and the “Valéry Perrier Race against ALS Fund.” M.L.H. and H.W. are supported by grants from the National Institute of Neurological Disorders and Stroke (NINDS) and National Institute of Aging (NIA) of the National Institute of Health (NIH) under the award numbers R01NS088645, RF1NS112719, R03AG064266, and R01NS094535, and the Houston Methodist Research Institute funds. M.L.H. holds the Everett E. and Randee K. Bernal Centennial Endowed Director of DNA Repair appointment at Houston Methodist. Publisher Copyright: © 2022 The Authors. Alzheimer's & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer's Association.
PY - 2022/8/22
Y1 - 2022/8/22
U2 - 10.1002/alz.12760
DO - 10.1002/alz.12760
M3 - Article
SN - 1552-5260
JO - Alzheimer's & Dementia: The Journal of the Alzheimer's Association
JF - Alzheimer's & Dementia: The Journal of the Alzheimer's Association
ER -