Projects per year
Abstract / Description of output
Biosecurity and sustainability in poultry production requires reliable germplasm conservation. Germplasm conservation in poultry is more challenging in comparison to other livestock species. Embryo cryopreservation is not feasible for egg-laying animals and chicken semen conservation has variable success for different chicken breeds. A potential solution is the cryopreservation of the committed diploid stem cell precursors to the gametes, the primordial germ cells (PGCs). Primordial germ cells are the lineage-restricted cells found at early embryonic stages in birds and form the sperm and eggs. We demonstrate here, using flocks of partially inbred lower fertility MHC restricted lines of chicken, that we can easily derive and cryopreserve a sufficient number of independent lines of male and female PGCs that would be sufficient to re-constitute a poultry breed. We demonstrate that germ line transmission can be attained from these PGCs using a commercial layer line of chickens as a surrogate host. This research is a major step in developing and demonstrating that cryopreserved PGCs could be used for the biobanking of specialised flocks of birds used in research settings. The prospective application of this technology to poultry production will further increase sustainability to meet current and future production needs.
Original language | English |
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Pages (from-to) | 1905-1911 |
Journal | Poultry Science |
Volume | 95 |
Issue number | 8 |
Early online date | 20 Apr 2016 |
DOIs | |
Publication status | E-pub ahead of print - 20 Apr 2016 |
Keywords / Materials (for Non-textual outputs)
- primordial germ cell
- chicken
- cryopreservation
- biobank
- stem cell
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Dive into the research topics of 'Cryopreservation of specialised Chicken Lines using cultured primordial germ cells'. Together they form a unique fingerprint.Projects
- 2 Finished
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Control of development and reproductive traits
Burdon, T., Argyle, D., Ashworth, C., Beard, P., Brunton, P., Burt, D., Clinton, M., Dunn, I., Farquharson, C., Headon, D., Hocking, P., Hohenstein, P., Hume, D., Jackson, I., McColl, B., McGrew, M., McLachlan, G., Sang, H., Summers, K. & Whitelaw, B.
1/04/12 → 31/03/17
Project: Research
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National avian research facility
Sang, H., Bernard, K., Hart, A., Kaiser, P., King, T. & Sherman, A.
1/04/12 → 31/03/17
Project: Research
Profiles
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Mike McGrew
- Royal (Dick) School of Veterinary Studies - Personal Chair of Avian Reproductive Technologies
Person: Academic: Research Active