Crystallization and preliminary diffraction studies of morphinone reductase, a flavoprotein involved in the degradation of morphine alkaloids

P C E Moody, N Shikotra, C E French, N C Bruce, N S Scrutton

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Abstract

Morphinone reductase from Pseudomonas putida M10, a flavoprotein involved in the degradation of morphine alkaloids, was purified from an overexpressing strain of Escherichia coli and crystallized using the hanging-drop vapour-diffusion method. Diffraction data were collected to 2.5 Angstrom. The I-centred orthorhombic cell has a monomer in the asymmetric unit. Preliminary molecular replacement calculations have been performed using Old Yellow Enzyme as the search model.

Original languageEnglish
Pages (from-to)619-621
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume53
DOIs
Publication statusPublished - 1 Sep 1997

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