Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

S L Pealing; D A Lysek; P Taylor; D Alexeev; G A Reid; S K Chapman; M D Walkinshaw

doi:10.1006/jsbi.1999.4139

Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

S L Pealing, D A Lysek, P Taylor, D Alexeev, G A Reid, S K Chapman, M D Walkinshaw

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The fumarate reductase (flavocytochrome c(3)) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 A, b = 92.107 A, c = 78.311 A, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 A. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P2(1) with cell dimensions of a = 45.359 A, b = 88.051 A, c = 77.473 A, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified.

Original language	English
Pages (from-to)	76-8
Number of pages	3
Journal	Journal of Structural Biology
Volume	127
Issue number	1
DOIs	https://doi.org/10.1006/jsbi.1999.4139
Publication status	Published - 1999

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title = "Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina",

abstract = "The fumarate reductase (flavocytochrome c(3)) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 A, b = 92.107 A, c = 78.311 A, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 A. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P2(1) with cell dimensions of a = 45.359 A, b = 88.051 A, c = 77.473 A, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified.",

author = "Pealing, {S L} and Lysek, {D A} and P Taylor and D Alexeev and Reid, {G A} and Chapman, {S K} and Walkinshaw, {M D}",

year = "1999",

doi = "10.1006/jsbi.1999.4139",

language = "English",

volume = "127",

pages = "76--8",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press",

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}

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

AU - Pealing, S L

AU - Lysek, D A

AU - Taylor, P

AU - Alexeev, D

AU - Reid, G A

AU - Chapman, S K

AU - Walkinshaw, M D

PY - 1999

Y1 - 1999

N2 - The fumarate reductase (flavocytochrome c(3)) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 A, b = 92.107 A, c = 78.311 A, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 A. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P2(1) with cell dimensions of a = 45.359 A, b = 88.051 A, c = 77.473 A, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified.

AB - The fumarate reductase (flavocytochrome c(3)) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 A, b = 92.107 A, c = 78.311 A, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 A. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P2(1) with cell dimensions of a = 45.359 A, b = 88.051 A, c = 77.473 A, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified.

U2 - 10.1006/jsbi.1999.4139

DO - 10.1006/jsbi.1999.4139

M3 - Article

C2 - 10479620

SN - 1047-8477

VL - 127

SP - 76

EP - 78

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 1

ER -

Crystallization and preliminary X-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

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