Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7

S S Sturrock, D T F Dryden, C Atanasiu, J Dornan, S Bruce, A Cronshaw, P Taylor, M D Walkinshaw

Research output: Contribution to journalArticlepeer-review

Abstract

Ocr, the product of gene 0.3 of bacteriophage T7, prevents the action of restriction endonucleases of the host bacteria. The amino-acid sequence of ocr has less than 20% similarity to any protein of known three-dimensional structure. Ocr has been crystallized in a number of different crystal forms and X-ray data for the seleno-L-methionine-substituted form has been collected to a resolution of 1.8 Angstrom. The presence of caesium was found to be required for good crystal growth. Anomalous X-ray data was used to identify possible positions for Se and Cs atoms in the unit cell.

Original languageEnglish
Pages (from-to)1652-1654
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue numberPart 11
DOIs
Publication statusPublished - Nov 2001

Keywords

  • ocr
  • gene 0.3
  • bacteriophage
  • anti-restriction

Fingerprint

Dive into the research topics of 'Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7'. Together they form a unique fingerprint.

Cite this