Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor

G Spraggon, D Stuart, C Ponting, C Finnis, D Sleep, Y Jones

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.

Original languageEnglish
Pages (from-to)879-80
Number of pages2
JournalJournal of Molecular Biology
Volume234
Issue number3
DOIs
Publication statusPublished - 5 Dec 1993

Keywords

  • Blood Platelets
  • Crystallization
  • Crystallography, X-Ray
  • Endothelial Growth Factors
  • Humans
  • Protein Conformation
  • Recombinant Proteins

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