Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor

G Spraggon; D Stuart; C Ponting; C Finnis; D Sleep; Y Jones

doi:10.1006/jmbi.1993.1632

Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor

G Spraggon, D Stuart, C Ponting, C Finnis, D Sleep, Y Jones

Deanery of Molecular, Genetic and Population Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.

Original language	English
Pages (from-to)	879-80
Number of pages	2
Journal	Journal of Molecular Biology
Volume	234
Issue number	3
DOIs	https://doi.org/10.1006/jmbi.1993.1632
Publication status	Published - 5 Dec 1993

Keywords

Blood Platelets
Crystallization
Crystallography, X-Ray
Endothelial Growth Factors
Humans
Protein Conformation
Recombinant Proteins

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10.1006/jmbi.1993.1632

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title = "Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor",

abstract = "Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.",

keywords = "Blood Platelets, Crystallization, Crystallography, X-Ray, Endothelial Growth Factors, Humans, Protein Conformation, Recombinant Proteins",

author = "G Spraggon and D Stuart and C Ponting and C Finnis and D Sleep and Y Jones",

year = "1993",

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day = "5",

doi = "10.1006/jmbi.1993.1632",

language = "English",

volume = "234",

pages = "879--80",

journal = "Journal of Molecular Biology",

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TY - JOUR

T1 - Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor

AU - Spraggon, G

AU - Stuart, D

AU - Ponting, C

AU - Finnis, C

AU - Sleep, D

AU - Jones, Y

PY - 1993/12/5

Y1 - 1993/12/5

N2 - Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.

AB - Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.

KW - Blood Platelets

KW - Crystallization

KW - Crystallography, X-Ray

KW - Endothelial Growth Factors

KW - Humans

KW - Protein Conformation

KW - Recombinant Proteins

U2 - 10.1006/jmbi.1993.1632

DO - 10.1006/jmbi.1993.1632

M3 - Article

C2 - 8254678

VL - 234

SP - 879

EP - 880

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

ER -

Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor

Abstract

Keywords

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