Cubic crystals of chloramphenicol phosphotransferase from Streptomyces venezuelae in complex with chloramphenicol

J Ellis, D J Campopiano, T Izard

Research output: Contribution to journalArticlepeer-review

Abstract

Chloramphenicol 3-O-phosphotransferase (CPT) from Streptomyces venezuelae ISP5230, a novel chloramphenicol-inactivating kinase, has been overexpressed and purified using Escherichia coli as the heterologous host. Crystals of CPT in complex with its substrate chloramphenicol (Cm) were obtained which were suitable for X-ray diffraction. The crystals belong to the cubic space group I4132 with unit-cell dimension a = 200.0 A. The initial CPT crystals diffracted to 3.5 A and the diffraction was improved significantly upon adding acetonitrile and Cm to the crystallization drop. The CPT-Cm crystals diffract to at least 2.8 A resolution.
Original languageEnglish
Pages (from-to)1086-8
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue numberPt 5
Publication statusPublished - 1999

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