Cutaneous hypersensitivity reactions to Psoroptes ovis and Der p 1 in sheep previously infested with P. ovis--the sheep scab mite

A H M van den Broek, J F Huntley, R E W Halliwell, J Machell, M Taylor, H R P Miller

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously shown that infestation with Psoroptes ovis induces an IgE response and intense tissue eosinophilia, typical of a Type I hypersensitivity response [Parasite Immunol. 22 (2000) 407]. Intradermal tests (IDSTs) suggest that there are also delayed and Arthus-type responses to this parasite. In order to study the nature of ovine cutaneous reactions to P. ovis, naïve controls and experimentally infested sheep (n = 5) were challenged intradermally with mite antigen. Challenge elicited immediate (P <0.001) and delayed (P <0.005) wheal reactions in sensitised sheep. At 6 (P <0.02) and 30 h (P <0.001) the predominant infiltrating cells were eosinophils. To explore the role of circulating antibodies, naïve sheep (n = 5) were subjected to Prausnitz-Kustner (PK) tests. These elicited immediate (P <0.02) but not delayed wheal reactions. At 6 h eosinophils (P <0.001) dominated the infiltrate. These results suggest that P. ovis allergens provoke an IgE-dependent immediate and late phase response and a cell-mediated eosinophil-rich delayed-type hypersensitivity response (ER-DTH).
Original languageEnglish
Pages (from-to)105-17
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume91
Issue number2
Publication statusPublished - 30 Jan 2003

Keywords

  • Animals
  • Antibodies
  • Antigens
  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Cysteine Endopeptidases
  • Granulocytes
  • Hypersensitivity
  • Leukocyte Count
  • Mast Cells
  • Mite Infestations
  • Psoroptidae
  • Sheep Diseases
  • Sheep, Domestic
  • Skin
  • Time Factors

Fingerprint

Dive into the research topics of 'Cutaneous hypersensitivity reactions to Psoroptes ovis and Der p 1 in sheep previously infested with P. ovis--the sheep scab mite'. Together they form a unique fingerprint.

Cite this