Abstract / Description of output
We investigated the influence of dendritic cell (DC) CD40 expression on Th2 and Th1 development by in vivo transfer of Ag-pulsed bone marrow-derived DC generated from wild-type (WT) or CD40(-/-) mice. Contrary to expectation, CD40(-/-) DC primed with Ag that inherently induce a Th2 response (soluble egg Ag from Schistosoma mansoni) failed to induce a Th2 response or any compensatory Th1 response, whereas CD40(-/-)DC primed with Ag that inherently induce a Th1 response (Propionibacterium acnes) generated a competent Th1 response. Thus, DC expression of CD40 is a prerequisite for initiation of Th2, but not Th1, responses by these Ag. Consistent with this, CD154(-/-) mice, unlike WT mice, failed to mount a Th2 response when directly injected with schistosome eggs but mounted a normal Th1 response after challenge with P. acnes. CD40-CD154 interaction can therefore play a major role in Th2 response induction.
| Original language | English |
|---|---|
| Pages (from-to) | 537-40 |
| Number of pages | 4 |
| Journal | The Journal of Immunology |
| Volume | 168 |
| Issue number | 2 |
| Publication status | Published - 2002 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Antigens, Bacterial
- Antigens, CD40
- Antigens, Helminth
- Bone Marrow Cells
- CD40 Ligand
- Cells, Cultured
- Cytokines
- Dendritic Cells
- Injections, Subcutaneous
- Lymphocyte Activation
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Ovum
- Propionibacterium acnes
- Schistosoma mansoni
- Th1 Cells
- Th2 Cells