Cutting edge: Th2 response induction by dendritic cells: a role for CD40

Andrew S MacDonald, Amy D Straw, Nicole M Dalton, Edward J Pearce

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the influence of dendritic cell (DC) CD40 expression on Th2 and Th1 development by in vivo transfer of Ag-pulsed bone marrow-derived DC generated from wild-type (WT) or CD40(-/-) mice. Contrary to expectation, CD40(-/-) DC primed with Ag that inherently induce a Th2 response (soluble egg Ag from Schistosoma mansoni) failed to induce a Th2 response or any compensatory Th1 response, whereas CD40(-/-)DC primed with Ag that inherently induce a Th1 response (Propionibacterium acnes) generated a competent Th1 response. Thus, DC expression of CD40 is a prerequisite for initiation of Th2, but not Th1, responses by these Ag. Consistent with this, CD154(-/-) mice, unlike WT mice, failed to mount a Th2 response when directly injected with schistosome eggs but mounted a normal Th1 response after challenge with P. acnes. CD40-CD154 interaction can therefore play a major role in Th2 response induction.
Original languageEnglish
Pages (from-to)537-40
Number of pages4
JournalJournal of Immunology
Volume168
Issue number2
Publication statusPublished - 2002

Keywords

  • Animals
  • Antigens, Bacterial
  • Antigens, CD40
  • Antigens, Helminth
  • Bone Marrow Cells
  • CD40 Ligand
  • Cells, Cultured
  • Cytokines
  • Dendritic Cells
  • Injections, Subcutaneous
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Ovum
  • Propionibacterium acnes
  • Schistosoma mansoni
  • Th1 Cells
  • Th2 Cells

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