CX(3)CR1(+) CD115(+) CD135(+) common macrophage/DC precursors and the role of CX(3)CR1 in their response to inflammation

Cedric Auffray, Darin K. Fogg, Emilie Narni-Mancinelli, Brigitte Senechal, Celine Trouillet, Noah Saederup, Julia Leemput, Karine Bigot, Laura Campisi, Marc Abitbol, Thierry Molina, Israel Charo, David A. Hume, Ana Cumano, Gregoire Lauvau, Frederic Geissmann

Research output: Contribution to journalArticlepeer-review

Abstract

CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1(+) inflammatory monocytes that differentiate into TipDCs during infection. CX(3)CR1 deficiency selectively impairs the recruitment of blood Gr1(+) monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.

Original languageEnglish
Pages (from-to)595-606
Number of pages12
JournalJournal of Experimental Medicine
Volume206
Issue number3
DOIs
Publication statusPublished - 16 Mar 2009

Keywords

  • Animals
  • Bone Marrow Cells/cytology
  • Bone Marrow Cells/immunology
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Cell Survival
  • Dendritic Cells/cytology
  • Dendritic Cells/enzymology
  • Dendritic Cells/immunology
  • Inflammation/enzymology
  • Inflammation/immunology
  • Listeria monocytogenes
  • Listeriosis/immunology
  • Macrophages/cytology
  • Macrophages/enzymology
  • Macrophages/immunology
  • Mice
  • Monocytes/cytology
  • Monocytes/immunology
  • Nitric Oxide Synthase Type II/metabolism
  • Phenotype
  • Reactive Oxygen Species/metabolism
  • Receptor, Macrophage Colony-Stimulating Factor/metabolism
  • Receptors, Chemokine/deficiency
  • Receptors, Chemokine/metabolism
  • Spleen/cytology
  • Spleen
  • Stem Cells
  • Tumor Necrosis Factor-alpha/biosynthesis
  • fms-Like Tyrosine Kinase 3/metabolism

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