Cytokine gene expression profiles in chicken spleen and intestinal tissues during Ascaridia galli infection

Janne Pleidrup A., Liselotte R. Norup, Tina S. Dalgaard, Pete Kaiser, Anders Permin, Torben W. Schou, Dorte Fink Vadekær, Gregers Jungersen, Poul Sørensen, Helle R. Juul-Madsen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In the poultry production industry, chickens with access to outdoor areas are exposed to a wide range of parasites e.g. the helminth Ascaridia galli. By real-time quantitative RT-PCR, the relative gene expression of the T helper 1 (Th1) cytokine IFN-γ, the T helper 2 (Th2) cytokine IL-13, the anti-inflammatory cytokines IL-10 and TGF-β4 and the pro-inflammatory cytokine IL-17F were determined over a period of 3 weeks in A. galli and non-A. galli-infected chickens. A characteristic Th2 response was observed in the jejunum of A. galli-infected chickens with increased expression of IL-13 and decreased expression of IFN-γ from day 14 post infection. At the putative time of larvae invasion into the intestinal mucosa (day 7), an increased expression of IFN-γ, IL-10, and TGF-β4 was observed in the spleen. At the putative onset of the innate immune response (day 10), a decreased expression of jejunal IFN-γ and IL-13 was observed. Finally, at the expected period of an adaptive immune response (days 14-21) a general decreased expression of IFN-γ and TGF-β4 in spleen and IFN-γ in jejunum was followed by a decreased expression of IFN-γ and IL-10 at day 21 in caecal tonsils.

Original languageEnglish
Pages (from-to)317-321
JournalVeterinary Parasitology
Issue number3-4
Publication statusPublished - 8 May 2014

Keywords / Materials (for Non-textual outputs)

  • A. galli
  • Chicken
  • Cytokines
  • Immune response


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