Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection

M. Vordermeier; G. Ameni; E.J. Glass

doi:10.1007/s11250-011-9950-x

Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection

M. Vordermeier, G. Ameni, E.J. Glass

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, we found that Holstein cattle (Bos taurus taurus) displayed higher skin test prevalence and disease severity compared to zebu (Bos taurus indicus) herd-mates kept under identical husbandry conditions in Ethiopia. To determine whether these susceptibility differences were patent at the level of the innate immune system, we infected monocytes from naive Holstein or Sahiwal zebu cattle with either live virulent Mycobacterium tuberculosis or Mycobacterium bovis. The cytokine profile following infection was compared between the two breeds by measuring IL-1alpha, IL-6, IL-10, IL-12 and TNF-alpha as well as nitric oxide in culture supernatants. Our results suggested subtle differences in the cytokine profile because only infection-induced IL-6 production was significantly increased in monocytes from the more susceptible Holstein cattle.

Original language	English
Pages (from-to)	651-655
Journal	Tropical Animal Health and Production
Volume	44
Issue number	3
Early online date	16 Aug 2011
DOIs	https://doi.org/10.1007/s11250-011-9950-x
Publication status	Published - 2012

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10.1007/s11250-011-9950-x

http://www.springerlink.com/content/4020071g00210563/

Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research
An Intergrated approach for the development of sustainable methods to control tropical theileriosis
Glass, E. (Principal Investigator)
UK-based charities
1/05/08 → 30/11/10
Project: Research

Cite this

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title = "Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection",

abstract = "Recently, we found that Holstein cattle (Bos taurus taurus) displayed higher skin test prevalence and disease severity compared to zebu (Bos taurus indicus) herd-mates kept under identical husbandry conditions in Ethiopia. To determine whether these susceptibility differences were patent at the level of the innate immune system, we infected monocytes from naive Holstein or Sahiwal zebu cattle with either live virulent Mycobacterium tuberculosis or Mycobacterium bovis. The cytokine profile following infection was compared between the two breeds by measuring IL-1alpha, IL-6, IL-10, IL-12 and TNF-alpha as well as nitric oxide in culture supernatants. Our results suggested subtle differences in the cytokine profile because only infection-induced IL-6 production was significantly increased in monocytes from the more susceptible Holstein cattle.",

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AB - Recently, we found that Holstein cattle (Bos taurus taurus) displayed higher skin test prevalence and disease severity compared to zebu (Bos taurus indicus) herd-mates kept under identical husbandry conditions in Ethiopia. To determine whether these susceptibility differences were patent at the level of the innate immune system, we infected monocytes from naive Holstein or Sahiwal zebu cattle with either live virulent Mycobacterium tuberculosis or Mycobacterium bovis. The cytokine profile following infection was compared between the two breeds by measuring IL-1alpha, IL-6, IL-10, IL-12 and TNF-alpha as well as nitric oxide in culture supernatants. Our results suggested subtle differences in the cytokine profile because only infection-induced IL-6 production was significantly increased in monocytes from the more susceptible Holstein cattle.

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JO - Tropical Animal Health and Production

JF - Tropical Animal Health and Production

IS - 3

ER -

Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection

Abstract

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Projects

Innate immunity and endemic diseases in livestock species

An Intergrated approach for the development of sustainable methods to control tropical theileriosis

Cite this